Development of strains of the thermotolerant yeast Hansenula polymorpha capable of alcoholic fermentation of starch and xylan

AY Voronovsky, OV Rohulya, CA Abbas… - Metabolic Engineering, 2009 - Elsevier
AY Voronovsky, OV Rohulya, CA Abbas, AA Sibirny
Metabolic Engineering, 2009Elsevier
The thermotolerant yeast Hansenula polymorpha ferments glucose and xylose to ethanol at
high temperatures. However, H. polymorpha cannot utilize starchy materials or xylans.
Heterologous amylolytic and xylanolytic enzymes have to be expressed in this yeast to
provide for utilization and growth on starch and xylan. Genes SWA2 and GAM1 from the
yeast Schwanniomyces occidentalis, encoding α-amylase and glucoamylase, respectively,
were expressed in H. polymorpha. The expression was achieved by integration of the SWA2 …
The thermotolerant yeast Hansenula polymorpha ferments glucose and xylose to ethanol at high temperatures. However, H. polymorpha cannot utilize starchy materials or xylans. Heterologous amylolytic and xylanolytic enzymes have to be expressed in this yeast to provide for utilization and growth on starch and xylan. Genes SWA2 and GAM1 from the yeast Schwanniomyces occidentalis, encoding α-amylase and glucoamylase, respectively, were expressed in H. polymorpha. The expression was achieved by integration of the SWA2 and GAM1 genes under the strong constitutive promoter of the H. polymorpha glyceraldehyde-3-phosphate dehydrogenase gene (HpGAP) into H. polymorpha genome. Resulting transformants acquired the ability to grow on a minimal medium containing soluble starch as a sole carbon source. Ethanol production at high-temperature fermentation from starch by the recombinant strains was up to 10g/L. The XYN2 gene encoding endoxylanase of the fungus Trichoderma reseei was expressed in H. polymorpha. Co-expression of xlnD gene coding for β-xylosidase of the fungus Aspergillus niger and the XYN2 gene in H. polymorpha was achieved by integration of these genes under control of the HpGAP promoter. Resulting transformants were capable of growth and alcoholic fermentation on a minimal medium supplemented with birchwood xylan as a sole carbon source at 48°C.
Elsevier
以上显示的是最相近的搜索结果。 查看全部搜索结果