Differential sensitivity of B lymphocyte populations to IgM receptor ligation is determined by local factors.
Y Modigliani, J Demengeot… - International …, 1997 - academic.oup.com
Y Modigliani, J Demengeot, R Vasconcellos, J Andersson, A Coutinho, A Grandien
International immunology, 1997•academic.oup.comLigation of surface IgM on B cells responding to lipopolysaccharide (LPS) suppresses
terminal differentiation and high-rate Ig secretion with no effect on proliferation. As shown
here, different B cell populations show characteristic mean values of ligand concentration
required for 50% inhibition, with Gaussian distributions of sensitivity to IgM receptor ligation
that reflect cellular heterogeneity of'al-or-none'inhibitions in single cells. Differential
sensitivity of B cell populations to IgM ligation seems to be locally determined by the cellular …
terminal differentiation and high-rate Ig secretion with no effect on proliferation. As shown
here, different B cell populations show characteristic mean values of ligand concentration
required for 50% inhibition, with Gaussian distributions of sensitivity to IgM receptor ligation
that reflect cellular heterogeneity of'al-or-none'inhibitions in single cells. Differential
sensitivity of B cell populations to IgM ligation seems to be locally determined by the cellular …
Abstract
Ligation of surface IgM on B cells responding to lipopolysaccharide (LPS) suppresses terminal differentiation and high-rate Ig secretion with no effect on proliferation. As shown here, different B cell populations show characteristic mean values of ligand concentration required for 50% inhibition, with Gaussian distributions of sensitivity to IgM receptor ligation that reflect cellular heterogeneity of 'al-or-none' inhibitions in single cells. Differential sensitivity of B cell populations to IgM ligation seems to be locally determined by the cellular environment and unrelated to the 'maturity' of the responding cells. Thus, while long-lived peritoneal B cells are 3- to 5-fold more resistant than splenic B cells, there is no difference in sensitivity between short- and long-lived B cells in the spleen. Furthermore, while B cells in bone marrow and spleen differ in sensitivity by two orders of magnitude, B cells differentiated in vitro from bone marrow pre-B cells are as resistant as splenic B cells. Moreover, bone marrow cell culture supernatants restore a high level of sensitivity in such cell populations. Differential sensitivity to IgM receptor ligation is reproduced by multivalent nominal antigen, in cell populations that show identical dose-response inhibition curves to direct activation of protein kinase C by phorbol esters. We conclude that the level of sensitivity to IgM ligation is independent of the life span or maturity of the B cell, but differentially regulated in vivo by putative tissue factors.
Oxford University Press
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