The evaluation of the motility data obtained with a CASA system, applying a Two-Step
Cluster analysis, identified in seabream sperm 3 different sperm subpopulations that
correlated differently with embryo hatching rates. Hence, we designed an experiment to
understand the effect of the application of different cryopreservation protocols in these sperm
motility-based subpopulations. We analyzed Sparus aurata frozen/thawed semen motility
15, 30, 45 and 60s after activation, using CASA software. Different protocols were applied for …