purpose. To determine the effect of latanoprost on the expression of human matrix
metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in the
trabecular meshwork (TM). methods. Total RNA was isolated, and qualitative RT-PCR was
performed to detect the mRNA of MMPs and TIMPs in human TM tissue and explant cultures
of TM endothelial cells. Cultures of TM cells were treated with vehicle control or latanoprost
acid for 24 hours. Real-time RT-PCR of cell cultures from five different donors was …

Objective To investigate the effect of topical latanoprost on the expression of matrix
metalloproteinases (MMPs) and tissue inhibitors of metalloproteinase 1 (TIMP-1) on the
ocular surface. Methods Tears were collected from 39 patients with glaucoma who used
latanoprost, 0.005%, eyedrops (Xalatan) and 28 healthy volunteers. The MMP-9
concentration was measured. Conjunctival epithelial cells were collected from 10 eyes of 10
patients before and 1 to 3 months after starting to take topical latanoprost, 0.005%, and MMP …