GLT‐1 down‐regulation induced by clozapine in rat frontal cortex is associated with synaptophysin up‐regulation
L Bragina, M Melone, G Fattorini… - Journal of …, 2006 - Wiley Online Library
Journal of neurochemistry, 2006•Wiley Online Library
In rat frontal cortex, extracellular levels of glutamate are raised by the anti‐psychotic drug
clozapine. We have recently shown that a significant reduction in the levels of the glutamate
transporter GLT‐1 may be one of the mechanisms responsible for this elevation. Here we
studied whether GLT‐1 down‐regulation induced by chronic clozapine treatment is
associated with changes in the expression of synaptophysin, synaptosome‐associated
protein of 25 kDa (SNAP‐25) and vesicular glutamate transporter 1 (VGLUT1), three major …
clozapine. We have recently shown that a significant reduction in the levels of the glutamate
transporter GLT‐1 may be one of the mechanisms responsible for this elevation. Here we
studied whether GLT‐1 down‐regulation induced by chronic clozapine treatment is
associated with changes in the expression of synaptophysin, synaptosome‐associated
protein of 25 kDa (SNAP‐25) and vesicular glutamate transporter 1 (VGLUT1), three major …
Abstract
In rat frontal cortex, extracellular levels of glutamate are raised by the anti‐psychotic drug clozapine. We have recently shown that a significant reduction in the levels of the glutamate transporter GLT‐1 may be one of the mechanisms responsible for this elevation. Here we studied whether GLT‐1 down‐regulation induced by chronic clozapine treatment is associated with changes in the expression of synaptophysin, synaptosome‐associated protein of 25 kDa (SNAP‐25) and vesicular glutamate transporter 1 (VGLUT1), three major presynaptic proteins involved in neurotransmitter release. Quantitative high‐resolution confocal microscopy studies in vivo showed that GLT‐1 down‐regulation is closely associated with a significant increase in synaptophysin, but not SNAP‐25 and VGLUT1, expression. This was confirmed in vitro studies, and in western blotting studies of synaptophysin, SNAP‐25 and VGLUT1. In addition, our results show that, following clozapine treatment, synaptophysin expression increases in the very cortical regions in which GLT‐1 expression is down‐regulated. These findings suggest that part of the effects of clozapine may be exerted via an action on the presynaptic machinery involved in neurotransmitter release.
Wiley Online Library
以上显示的是最相近的搜索结果。 查看全部搜索结果