Abstract Ischemia/reperfusion (I/R) leads to Acute Kidney Injury. HIF-1α is a key factor during
organ response to I/R. We previously demonstrated that HIF-1α is induced during renal
reperfusion, after ischemia. Here we investigate the role of HIF-1α and the HIF-1α
dependent mechanisms in renal repair after ischemia. By interference of HIF-1α in a rat
model of renal I/R, we observed loss of expression and mis-localization of e-cadherin and
induction of α-SMA, MMP-13, TGFβ, and collagen I. Moreover, we demonstrate that HIF-1α …

Results Previous data of our lab demonstrated that HIF-1α is stabilized in proximal tubule
cells during ischemia and in late reperfusion. Moreover in vivo interference of HIF-1α leads
to an exacerbated tissue damage and renal dysfunction3. In order to study the role and the
mechanisms triggered by HIF-1α in I/R injury resolution, we efficiently interfered the HIF-1α
protein and reduced HIF-1α activity in our model of renal I/R in the present work
(Supplementary Figures S1 and S2). HIF-1α interference during reperfusion increased …