Herminiimonas contaminans sp. nov., isolated as a contaminant of biopharmaceuticals
P Kämpfer, S P. Glaeser, N Lodders… - … of systematic and …, 2013 - microbiologyresearch.org
P Kämpfer, S P. Glaeser, N Lodders, HJ Busse, E Falsen
International journal of systematic and evolutionary …, 2013•microbiologyresearch.orgA Gram-staining-negative, rod-shaped, non-spore-forming bacterium isolated as a
contaminant from a biopharmaceutical process (strain CCUG 53591T) was studied for its
taxonomic allocation. On the basis of 16S rRNA gene sequence similarity data, this strain
was clearly allocated to the genus Herminiimonas. Herminiimonas saxobsidens was shown
to be the most closely related species on the basis of 16S rRNA gene sequence similarity
(99.9%), followed by Herminiimonas glaciei (99.6%) and Herminiimonas arsenicoxydans …
contaminant from a biopharmaceutical process (strain CCUG 53591T) was studied for its
taxonomic allocation. On the basis of 16S rRNA gene sequence similarity data, this strain
was clearly allocated to the genus Herminiimonas. Herminiimonas saxobsidens was shown
to be the most closely related species on the basis of 16S rRNA gene sequence similarity
(99.9%), followed by Herminiimonas glaciei (99.6%) and Herminiimonas arsenicoxydans …
A Gram-staining-negative, rod-shaped, non-spore-forming bacterium isolated as a contaminant from a biopharmaceutical process (strain CCUG 53591T) was studied for its taxonomic allocation. On the basis of 16S rRNA gene sequence similarity data, this strain was clearly allocated to the genus Herminiimonas . Herminiimonas saxobsidens was shown to be the most closely related species on the basis of 16S rRNA gene sequence similarity (99.9 %), followed by Herminiimonas glaciei (99.6 %) and Herminiimonas arsenicoxydans (98.8 %). Strain ND5, previously reported as H. glaciei , but showing 100 % 16S rRNA gene sequence similarity to strain CCUG 53591T, was included in the comparative study. Similarities to all other species of the genus Herminiimonas were below 98.0 %. Chemotaxonomic data (major ubiquinone, Q-8; major polar lipids, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; and major fatty acids, C17 : 0 cyclo, C19 : 0 cyclo ω8c and C16 : 0, with C10 : 0 3-OH as hydroxylated fatty acid) supported the affiliation of the isolate to the genus Herminiimonas . DNA–DNA hybridization results (mean values) for strain CCUG 53591T with H. saxobsidens CCUG 59860T (34 %), H. glaciei DSM 21140T (57 %), H. arsenicoxydans DSM 17148T (17 %) and Herminiimonas fonticola S-94T (11 %) clearly supported the separate taxonomic position of this strain. Strain ND5 showed DNA–DNA similarities of 78, 56 and 52 % to strain CCUG 53591T, H. glaciei DSM 21140T and H. saxobsidens CCUG 59860T, respectively. Phenotypic differentiation of the isolate from the most closely related species was possible by various features. Hence, strain CCUG 53591T represents a novel species, for which the name Herminiimonas contaminans sp. nov. is proposed, with the type strain CCUG 53591T ( = CCM 7991T). Strain ND5 is a second strain of this species.
Microbiology Research
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