In vivo nuclear magnetic resonance analysis of immobilization effects on glucose metabolism of yeast Saccharomyces cerevisiae
JL Galazzo, JE Bailey - Biotechnology and bioengineering, 1989 - Wiley Online Library
JL Galazzo, JE Bailey
Biotechnology and bioengineering, 1989•Wiley Online LibraryFermentation rates and intracellular compositions have been determined for alginate‐
entrapped Saccharomyces cerevisiae and for identical cells in suspension. Glucose uptake
and ethanol and glycerol production are approximately two times faster in immobilized cells
than in suspended cells. Phosphorus‐31 nuclear magnetic resonance (NMR) spectroscopy
of fermenting immobilized and suspended cells shows differences in intermediate
metabolite levels such as fructose‐1, 6 diphosphate, glucose‐6‐phosphate, and 3 …
entrapped Saccharomyces cerevisiae and for identical cells in suspension. Glucose uptake
and ethanol and glycerol production are approximately two times faster in immobilized cells
than in suspended cells. Phosphorus‐31 nuclear magnetic resonance (NMR) spectroscopy
of fermenting immobilized and suspended cells shows differences in intermediate
metabolite levels such as fructose‐1, 6 diphosphate, glucose‐6‐phosphate, and 3 …
Abstract
Fermentation rates and intracellular compositions have been determined for alginate‐entrapped Saccharomyces cerevisiae and for identical cells in suspension. Glucose uptake and ethanol and glycerol production are approximately two times faster in immobilized cells than in suspended cells. Phosphorus‐31 nuclear magnetic resonance (NMR) spectroscopy of fermenting immobilized and suspended cells shows differences in intermediate metabolite levels such as fructose‐1,6 diphosphate, glucose‐6‐phosphate, and 3‐phosphoglycerate and in internal pH. Carbon‐13 NMR shows an increase in polysaccharide production. These data suggest that immobilization has accelerated the rate of glucose transport or of glucose phosphorylation. These effects of immobilization upon cell metabolism are observed in a very short period of time under conditions in which negligible DNA, RNA, or protein synthesis takes place.
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