Independent regulation of ABCB1 and ABCC activities in thymocytes and bone marrow mononuclear cells during aging

F Kyle‐Cezar, J Echevarria‐Lima… - … journal of immunology, 2007 - Wiley Online Library
F Kyle‐Cezar, J Echevarria‐Lima, VM Rumjanek
Scandinavian journal of immunology, 2007Wiley Online Library
Aging modifies a number of functional and phenotypic parameters of cells from the immune
system. In this study, the activities of two members of the superfamily of ATP‐binding
cassette (ABC) transport proteins, ABCB1 and ABCC (measured by rhodamine 123 efflux
and Fluo‐3 efflux respectively), were compared in murine bone marrow cells and
thymocytes of young (3–4 weeks old), adult (2–3 months old) and old (18 months old) mice.
ABCB1 activity was shown to be age regulated in murine bone marrow mononuclear cells …
Abstract
Aging modifies a number of functional and phenotypic parameters of cells from the immune system. In this study, the activities of two members of the superfamily of ATP‐binding cassette (ABC) transport proteins, ABCB1 and ABCC (measured by rhodamine 123 efflux and Fluo‐3 efflux respectively), were compared in murine bone marrow cells and thymocytes of young (3–4 weeks old), adult (2–3 months old) and old (18 months old) mice. ABCB1 activity was shown to be age regulated in murine bone marrow mononuclear cells and thymocytes. In the bone marrow, the increased amount of cells with ABCB1 activity observed in old mice was restricted to the c‐kitSca‐1+ and c‐kit+Sca‐1+ subpopulations. Only a small percentage of c‐kit+ cells in the thymus had ABCB1 activity, and this subpopulation increased with age. In the thymus, old age augmented this activity in the CD4 CD8 double‐negative cells and in the CD4+ and CD8+ single‐positive populations. The activity of another ABC transporter, the ABCC‐related activity, was also modified by age in the bone marrow. However, the age‐related increase was observed in the subpopulations were ABCB1 was not modified, namely the non‐progenitor population (c‐kitSca‐1cells) and c‐kit+Sca‐1 cells. Nearly, all thymocytes expressed the ABCC1 molecule in an active form and aging did not affect this pattern. This study demonstrates an independent upregulation of ABCB1 and ABCC activities during the aging process. The increases were observed in different subsets of cells but followed a developmentally regulated pattern. The functions played by these transporters and alterations in aging are discussed.
Wiley Online Library
以上显示的是最相近的搜索结果。 查看全部搜索结果