KIF3A regulates the Wnt/β-catenin pathway via transporting β-catenin during spermatogenesis in Eriocheir sinensis
YJ Liang, JH Ni, LM Wang, FQ Tan, WX Yang - Cell and tissue research, 2020 - Springer
YJ Liang, JH Ni, LM Wang, FQ Tan, WX Yang
Cell and tissue research, 2020•SpringerThe Wnt/β-catenin pathway participates in many important physiological events such as cell
proliferation and differentiation in the male reproductive system. We found that Kinesin-2
motor KIF3A is highly expressed during spermatogenesis in Eriocheir sinensis; it may
potentially promote the intracellular transport of cargoes in this process. However, only a few
studies have focused on the relationship between KIF3A and the Wnt/β-catenin pathway in
the male reproductive system of decapod crustaceans. In this study, we cloned and …
proliferation and differentiation in the male reproductive system. We found that Kinesin-2
motor KIF3A is highly expressed during spermatogenesis in Eriocheir sinensis; it may
potentially promote the intracellular transport of cargoes in this process. However, only a few
studies have focused on the relationship between KIF3A and the Wnt/β-catenin pathway in
the male reproductive system of decapod crustaceans. In this study, we cloned and …
Abstract
The Wnt/β-catenin pathway participates in many important physiological events such as cell proliferation and differentiation in the male reproductive system. We found that Kinesin-2 motor KIF3A is highly expressed during spermatogenesis in Eriocheir sinensis; it may potentially promote the intracellular transport of cargoes in this process. However, only a few studies have focused on the relationship between KIF3A and the Wnt/β-catenin pathway in the male reproductive system of decapod crustaceans. In this study, we cloned and characterized the CDS of β-catenin in E. sinensis for the first time. Fluorescence in situ hybridization and immunofluorescence results showed the colocalization of Es-KIF3A and Es-β-catenin at the mRNA and the protein level respectively. To further explore the regulatory function of Es-KIF3A to the Wnt/β-catenin pathway, the es-kif3a was knocked down by double-stranded RNA (dsRNA) in vivo and in primary cultured cells in testes of E. sinensis. Results showed that the expression of es-β-catenin and es-dvl were decreased in the es-kif3a knockdown group. The protein expression level of Es-β-catenin was also reduced and the location of Es-β-catenin was changed from nucleus to cytoplasm in the late stage of spermatogenesis when es-kif3a was knocked down. Besides, the co-IP result demonstrated that Es-KIF3A could bind with Es-β-catenin. In summary, this study indicates that Es-KIF3A can positively regulate the Wnt/β-catenin pathway during spermatogenesis and Es-KIF3A can bind with Es-β-catenin to facilitate the nuclear translocation of Es-β-catenin.
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