Structures of ternary complexes of rat DNA polymerase β, a DNA template-primer, and ddCTP

H Pelletier, MR Sawaya, A Kumar, SH Wilson, J Kraut - Science, 1994 - science.org
… Although many auxiliary proteins such as ligases, helicases, … we were unable to see the
Mg2+ ions in electron density … , perhaps the nucleotidyl transfer mechanism that we present …

A ribozyme that triphosphorylates RNA 5′-hydroxyl groups

JE Moretti, UF Müller - Nucleic acids research, 2014 - academic.oup.com
… most active clones from the ligase ribozyme-based assay. … The fastest rate is obtained at 400
mM free [Mg2+]. The half-… Mg 2+ ions accelerate the uncatalyzed reaction (63) but no kinetic

Structure and mechanism of CutA, RNA nucleotidyl transferase with an unusual preference for cytosine

D Malik, K Kobyłecki, P Krawczyk… - Nucleic acids …, 2020 - academic.oup.com
… against RNAs that terminate with guanosine and catalysis of … placed on ice and 1 μl of T4
RNA Ligase 2, Truncated (200 U/… The Mg2+ ion is shown as a green sphere. (C) Binding of the …

Enzymatic properties of rat DNA polymerase β mutants obtained by randomized mutagenesis

A Skandalis, LA Loeb - Nucleic acids research, 2001 - academic.oup.com
… C-terminal domain with nucleotidyl transfer activity and an 8 … gel purified and ligated using
T4 DNA ligase into a ClaI/SphI … The purple spheres represent the Mg2+ ions. This figure was …