Label-free dynamic imaging of mitochondria and lysosomes within living cells via simultaneous dual-pump photothermal microscopy
J Miyazaki, Y Toumon - Biomedical Optics Express, 2019 - opg.optica.org
The dynamic activities of mitochondria and lysosomes, which play important roles in
maintaining cellular homeostasis, were observed without labeling by using highly sensitive
photothermal (PT) microscopy. This imaging modality allows for the direct observation of
cellular organelles that contain endogenous chromophores, with high temporal and spatial
resolution. We identified mitochondria and lysosomes inside living mammalian cells via
simultaneous dual-color imaging. Moreover, dynamic imaging revealed that the lysosomes …
maintaining cellular homeostasis, were observed without labeling by using highly sensitive
photothermal (PT) microscopy. This imaging modality allows for the direct observation of
cellular organelles that contain endogenous chromophores, with high temporal and spatial
resolution. We identified mitochondria and lysosomes inside living mammalian cells via
simultaneous dual-color imaging. Moreover, dynamic imaging revealed that the lysosomes …
