Light-driven amino acid uptake in Streptococcus cremoris or Clostridium acetobutylicum membrane vesicles fused with liposomes containing bacterial reaction …
W Crielaard, AJ Driessen, D Molenaar… - Journal of …, 1988 - Am Soc Microbiol
Journal of bacteriology, 1988•Am Soc Microbiol
Reaction centers of the phototrophic bacterium Rhodopseudomonas palustris were
introduced as proton motive force-generating systems in membrane vesicles of two
anaerobic bacteria. Liposomes containing reaction center-light-harvesting complex I
pigment protein complexes were fused with membrane vesicles of Streptococcus cremoris
or Clostridium acetobutylicum by freeze-thawing and sonication. Illumination of these fused
membranes resulted in the generation of a proton motive force of approximately-110 mV …
introduced as proton motive force-generating systems in membrane vesicles of two
anaerobic bacteria. Liposomes containing reaction center-light-harvesting complex I
pigment protein complexes were fused with membrane vesicles of Streptococcus cremoris
or Clostridium acetobutylicum by freeze-thawing and sonication. Illumination of these fused
membranes resulted in the generation of a proton motive force of approximately-110 mV …
Reaction centers of the phototrophic bacterium Rhodopseudomonas palustris were introduced as proton motive force-generating systems in membrane vesicles of two anaerobic bacteria. Liposomes containing reaction center-light-harvesting complex I pigment protein complexes were fused with membrane vesicles of Streptococcus cremoris or Clostridium acetobutylicum by freeze-thawing and sonication. Illumination of these fused membranes resulted in the generation of a proton motive force of approximately -110 mV. The magnitude of the proton motive force in these membranes could be varied by changing the light intensity. As a result of this proton motive force, amino acid transport into the fused membranes could be observed. The initial rate of leucine transport by membrane vesicles of S. cremoris increased exponentially with the proton motive force. An H+/leucine stoichiometry of 0.8 was determined from the steady-state level of leucine accumulation and the proton motive force, and this stoichiometry was found to be independent of the magnitude of the proton motive force. These results indicate that the introduction of bacterial reaction centers in membrane vesicles by the fusion procedure yields very attractive model systems for the study of proton motive force-consuming processes in membrane vesicles of (strict) anaerobic bacteria.
American Society for Microbiology
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