Localized monocyte chemotactic protein-1 production correlates with T cell infiltration of synovium in patients with psoriatic arthritis.
EL Ross, D D'Cruz, WJ Morrow - The Journal of rheumatology, 2000 - europepmc.org
EL Ross, D D'Cruz, WJ Morrow
The Journal of rheumatology, 2000•europepmc.orgObjective To examine normal and psoriatic skin and synovial tissue from patients with
psoriatic arthritis (PsA) for evidence of monocyte chemotactic protein-1 (MCP-1) mediated T
cell chemotaxis. Methods Peripheral blood (PB), synovial fluid (SF), normal and psoriatic
skin, and synovial biopsies were obtained from patients with PsA (n= 19) and compared to
samples from normal (n= 5) and disease (n= 5) controls (NC, DC). Immune cell populations
in PB and SF samples were assessed by immunofluorescent labeling and flow cytometry …
psoriatic arthritis (PsA) for evidence of monocyte chemotactic protein-1 (MCP-1) mediated T
cell chemotaxis. Methods Peripheral blood (PB), synovial fluid (SF), normal and psoriatic
skin, and synovial biopsies were obtained from patients with PsA (n= 19) and compared to
samples from normal (n= 5) and disease (n= 5) controls (NC, DC). Immune cell populations
in PB and SF samples were assessed by immunofluorescent labeling and flow cytometry …
Objective
To examine normal and psoriatic skin and synovial tissue from patients with psoriatic arthritis (PsA) for evidence of monocyte chemotactic protein-1 (MCP-1) mediated T cell chemotaxis.
Methods
Peripheral blood (PB), synovial fluid (SF), normal and psoriatic skin, and synovial biopsies were obtained from patients with PsA (n= 19) and compared to samples from normal (n= 5) and disease (n= 5) controls (NC, DC). Immune cell populations in PB and SF samples were assessed by immunofluorescent labeling and flow cytometry, levels of soluble MCP-1 were determined by quantitative ELISA, and immunohistochemistry was used to detect T cell subsets and macrophages and MCP-1 protein in frozen skin and synovial tissue sections.
Results
CD8+ but not CD4+ T cells were elevated in SF compared to PB, and the majority of these cells expressed CD45RO. Plasma MCP-1 levels in PsA were elevated relative to NC. MCP-1 levels were significantly higher than paired plasma samples in patients with recent onset (< 6 mo) synovitis (n= 10). A positive correlation was observed between synovial T cell numbers and MCP-1 levels in SF. MCP-1 protein was present in all tissues examined, but most intense expression was observed in synovium.
Conclusion
Elevated concentrations of MCP-1 concomitant with memory T cell infiltration in PsA SF suggests that MCP-1 mediated chemotaxis is involved in the recruitment of T lymphocytes into the synovial compartment of patients with PsA.
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