Marinilabilia nitratireducens sp. nov., a lipolytic bacterium of the family Marinilabiliaceae isolated from marine solar saltern

S Shalley, S Pradip Kumar, TNR Srinivas… - Antonie van …, 2013 - Springer
Antonie van Leeuwenhoek, 2013Springer
A Gram-negative, rod shaped, motile bacterium, was isolated from a marine solar saltern
sample collected from Kakinada, India. Strain AK2 T was determined to be positive for nitrate
reduction, catalase, Ala-Phe-Pro-arylamidase, β-galactosidase, β-N-acetylglucosaminidase,
β-glucosidase, β-xylosidase, α-glucosidase, α-galactosidase and phosphatase activities,
hydrolysis of aesculin, Tween 20/40/60/80 and urea. It was determined to be negative for
oxidase, lysine decarboxylase and ornithine decarboxylase activities and could not …
Abstract
A Gram-negative, rod shaped, motile bacterium, was isolated from a marine solar saltern sample collected from Kakinada, India. Strain AK2T was determined to be positive for nitrate reduction, catalase, Ala-Phe-Pro-arylamidase, β-galactosidase, β-N-acetylglucosaminidase, β-glucosidase, β-xylosidase, α-glucosidase, α-galactosidase and phosphatase activities, hydrolysis of aesculin, Tween 20/40/60/80 and urea. It was determined to be negative for oxidase, lysine decarboxylase and ornithine decarboxylase activities and could not hydrolyze agar, casein, gelatin and starch. The predominant fatty acids were identified as iso-C15:0 (28.2 %), anteiso-C15:0 (23.2 %), iso-C13:0 (19.9 %) and iso-C15:0 3-OH (13.9 %). Strain AK2T was found to contain menaquinone with seven isoprene units (MK-7) as the sole respiratory quinone and phosphatidylethanolamine, one unidentified phospholipid and three unidentified lipids as polar lipids. The 16S rRNA gene sequence analysis indicated the strain AK2T as a member of the genus Marinilabilia and is closely related to Marinilabilia salmonicolor with pair-wise sequence similarity of 98.2 %. Phylogenetic analysis of 16S rRNA gene revealed that the strain AK2T clustered with M. salmonicolor. However, DNA–DNA hybridization with M. salmonicolor JCM 21150T showed a relatedness of 48 ± 0.5 % with respect to strain AK2T. The DNA G+C content of the strain was determined to be 40.2 mol%. Based on the phenotypic characteristics and phylogenetic inference, it is proposed that the strain AK2T represents a novel species of the genus Marinilabilia, for which the name Marinilabilia nitratireducens sp. nov. is proposed. The type strain of M. nitratireducens sp. nov. is AK2T (= MTCC 11402T = JCM 17679T).
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