Measurement of 5-aminolaevulinate synthase activity by gas-liquid chromatography with electron-capture detection

A Gorchein - Biochemical Journal, 1990 - portlandpress.com
A Gorchein
Biochemical Journal, 1990portlandpress.com
A specific assay for 5-aminolaevulinate synthase activity is described, with a sensitivity
comparable with that of radiochemical assays. It is based on measurement by glc with
electron-capture detection of the pentafluorobenzyl ester of the ethyl acetoacetate pyrrole
derivative of 5-aminolaevulinic acid, and of the corresponding compound from 6-amino-5-
oxohexanoic acid used as internal standard. Enzyme activity has been measured in
homogenates of rat liver, spleen, kidney and brain, and in human lymphocytes.
A specific assay for 5-aminolaevulinate synthase activity is described, with a sensitivity comparable with that of radiochemical assays. It is based on measurement by g.l.c. with electron-capture detection of the pentafluorobenzyl ester of the ethyl acetoacetate pyrrole derivative of 5-aminolaevulinic acid, and of the corresponding compound from 6-amino-5-oxohexanoic acid used as internal standard. Enzyme activity has been measured in homogenates of rat liver, spleen, kidney and brain, and in human lymphocytes.
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