Measurement of 5-aminolaevulinate synthase activity by gas-liquid chromatography with electron-capture detection
A Gorchein - Biochemical Journal, 1990 - portlandpress.com
A Gorchein
Biochemical Journal, 1990•portlandpress.comA specific assay for 5-aminolaevulinate synthase activity is described, with a sensitivity
comparable with that of radiochemical assays. It is based on measurement by glc with
electron-capture detection of the pentafluorobenzyl ester of the ethyl acetoacetate pyrrole
derivative of 5-aminolaevulinic acid, and of the corresponding compound from 6-amino-5-
oxohexanoic acid used as internal standard. Enzyme activity has been measured in
homogenates of rat liver, spleen, kidney and brain, and in human lymphocytes.
comparable with that of radiochemical assays. It is based on measurement by glc with
electron-capture detection of the pentafluorobenzyl ester of the ethyl acetoacetate pyrrole
derivative of 5-aminolaevulinic acid, and of the corresponding compound from 6-amino-5-
oxohexanoic acid used as internal standard. Enzyme activity has been measured in
homogenates of rat liver, spleen, kidney and brain, and in human lymphocytes.
A specific assay for 5-aminolaevulinate synthase activity is described, with a sensitivity comparable with that of radiochemical assays. It is based on measurement by g.l.c. with electron-capture detection of the pentafluorobenzyl ester of the ethyl acetoacetate pyrrole derivative of 5-aminolaevulinic acid, and of the corresponding compound from 6-amino-5-oxohexanoic acid used as internal standard. Enzyme activity has been measured in homogenates of rat liver, spleen, kidney and brain, and in human lymphocytes.
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