Mechanistic Differences in DNA Nanoparticle Formation in the Presence of Oligolysines and Poly-l-lysine
I Nayvelt, T Thomas, TJ Thomas - Biomacromolecules, 2007 - ACS Publications
I Nayvelt, T Thomas, TJ Thomas
Biomacromolecules, 2007•ACS PublicationsWe studied the effectiveness of trilysine (Lys3), tetralysine (Lys4), pentalysine (Lys5), and
poly-l-lysine (PLL)(MW 50000) on λ-DNA nanoparticle formation and characterized the size,
shape, and stability of nanoparticles. Light scattering experiments showed EC50 (lysine
concentration at 50% DNA compaction) values of∼ 0.0036, 2, and 20 μmol/L, respectively,
for PLL, Lys5, and Lys4 at 10 mM [Na+]. Plots of log EC50 versus log [Na+] showed positive
slopes of 1.09 and 1.7, respectively, for Lys4 and Lys5 and a negative slope of− 0.1 for PLL …
poly-l-lysine (PLL)(MW 50000) on λ-DNA nanoparticle formation and characterized the size,
shape, and stability of nanoparticles. Light scattering experiments showed EC50 (lysine
concentration at 50% DNA compaction) values of∼ 0.0036, 2, and 20 μmol/L, respectively,
for PLL, Lys5, and Lys4 at 10 mM [Na+]. Plots of log EC50 versus log [Na+] showed positive
slopes of 1.09 and 1.7, respectively, for Lys4 and Lys5 and a negative slope of− 0.1 for PLL …
We studied the effectiveness of trilysine (Lys3), tetralysine (Lys4), pentalysine (Lys5), and poly-l-lysine (PLL) (MW 50000) on λ-DNA nanoparticle formation and characterized the size, shape, and stability of nanoparticles. Light scattering experiments showed EC50 (lysine concentration at 50% DNA compaction) values of ∼0.0036, 2, and 20 μmol/L, respectively, for PLL, Lys5, and Lys4 at 10 mM [Na+]. Plots of log EC50 versus log [Na+] showed positive slopes of 1.09 and 1.7, respectively, for Lys4 and Lys5 and a negative slope of −0.1 for PLL. Hydrodynamic radii of oligolysine condensed particles increased (48−173 nm) with increasing [Na+], whereas no significant change occurred to nanoparticles formed with PLL. There was an increase in the size of nanoparticles formed with Lys5 at >40 °C, whereas no such change occurred with PLL. The DNA melting temperature increased with oligolysine concentration. These results indicate distinct differences in the mechanism(s) by which oligolysines and PLL provoke DNA condensation to nanoparticles.
ACS Publications
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