We studied the effectiveness of trilysine (Lys₃), tetralysine (Lys₄), pentalysine (Lys₅), and poly-l-lysine (PLL) (MW 50000) on λ-DNA nanoparticle formation and characterized the size, shape, and stability of nanoparticles. Light scattering experiments showed EC₅₀ (lysine concentration at 50% DNA compaction) values of ∼0.0036, 2, and 20 μmol/L, respectively, for PLL, Lys₅, and Lys₄ at 10 mM [Na⁺]. Plots of log EC₅₀ versus log [Na⁺] showed positive slopes of 1.09 and 1.7, respectively, for Lys₄ and Lys₅ and a negative slope of −0.1 for PLL. Hydrodynamic radii of oligolysine condensed particles increased (48−173 nm) with increasing [Na⁺], whereas no significant change occurred to nanoparticles formed with PLL. There was an increase in the size of nanoparticles formed with Lys₅ at >40 °C, whereas no such change occurred with PLL. The DNA melting temperature increased with oligolysine concentration. These results indicate distinct differences in the mechanism(s) by which oligolysines and PLL provoke DNA condensation to nanoparticles.