[HTML][HTML] Metal ion dependence of DNA cleavage by SepMI and EhoI restriction endonucleases
A Belkebir, H Azeddoug - Microbiological research, 2013 - Elsevier
Most of type II restriction endonucleases show an absolute requirement for divalent metal
ions as cofactors for DNA cleavage. While Mg2+ is the natural cofactor other metal ions can
substitute it and mediate the catalysis, however Ca2+ (alone) only supports DNA binding. To
investigate the role of Mg2+ in DNA cleavage by restriction endonucleases, we have studied
the Mg2+ and Mn2+ concentration dependence of DNA cleavage by SepMI and EhoI.
Digestion reactions were carried out at different Mg2+ and Mn2+ concentrations at constant …
ions as cofactors for DNA cleavage. While Mg2+ is the natural cofactor other metal ions can
substitute it and mediate the catalysis, however Ca2+ (alone) only supports DNA binding. To
investigate the role of Mg2+ in DNA cleavage by restriction endonucleases, we have studied
the Mg2+ and Mn2+ concentration dependence of DNA cleavage by SepMI and EhoI.
Digestion reactions were carried out at different Mg2+ and Mn2+ concentrations at constant …
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