Methanobacterium aarhusense sp. nov., a novel methanogen isolated from a marine sediment (Aarhus Bay, Denmark)

AG Shlimon, MW Friedrich… - … of systematic and …, 2004 - microbiologyresearch.org
International journal of systematic and evolutionary …, 2004microbiologyresearch.org
Strain H2-LRT, a 5–18 μm long and 0· 7 μm wide filamentous, mesophilic, moderately
halophilic, non-motile hydrogenotrophic methanogen, was isolated from marine sediment of
Aarhus Bay, Denmark, 1· 7 m below the sediment surface. On the basis of 16S rRNA gene
comparison with sequences of known methanogens, strain H2-LRT could be affiliated to the
genus Methanobacterium. The strain forms a distinct line of descent within this genus, with
Methanobacterium oryzae (95· 9% sequence identity) and Methanobacterium bryantii (95 …
Strain H2-LRT, a 5–18 μm long and 0·7 μm wide filamentous, mesophilic, moderately halophilic, non-motile hydrogenotrophic methanogen, was isolated from marine sediment of Aarhus Bay, Denmark, 1·7 m below the sediment surface. On the basis of 16S rRNA gene comparison with sequences of known methanogens, strain H2-LRT could be affiliated to the genus Methanobacterium. The strain forms a distinct line of descent within this genus, with Methanobacterium oryzae (95·9 % sequence identity) and Methanobacterium bryantii (95·7 % sequence identity) as its closest relatives. The 16S rRNA-based affiliation was supported by comparison of the mcrA gene, which encodes the α-subunit of methyl-coenzyme M reductase. Strain H2-LRT grew only on H2/CO2. The DNA G+C content is 34·9 mol%. Optimum growth temperature was 45 °C. The strain grew equally well at pH 7·5 and 8. No growth or methane production was observed below pH 5 or above pH 9. Strain H2-LRT grew well within an NaCl concentration range of 100 and 900 mM. No growth or methane production was observed at 1 M NaCl. At 50 mM NaCl, growth and methane production were reduced. Based on 16S rRNA gene sequence analysis, the isolate is proposed to represent a novel taxon within the genus Methanobacterium, namely Methanobacterium aarhusense sp. nov. The type strain is H2-LRT (=DSM 15219T=ATCC BAA-828T).
Microbiology Research
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