In this research, the creation of optimum conditions for the formation of sodium hyaluronate-TiO₂ bionanocomposite and its antibacterial effect on gram positive and gram negative bacteria was evaluated. The Fourier transform infrared spectroscopy spectra, scanning electron microscopy images and energy dispersive X-ray spectroscopy pattern confirmed the formation of the bionanocomposite. Thermogravimetric analysis and differential thermal analysis indicated that the thermal stability rate had significantly improved with formation of the bionanocomposite. Nine experiments were designed based on the Taguchi method by applying different proportions of sodium hyaluronate biopolymer and TiO₂ nanoparticles at different stirring times. Bionanocomposite produced under conditions of experiment 5 (TiO₂ 4 mg/ml, sodium hyaluronate 1 mg/ml and stirring time of 90 min) and experiment 9 (TiO₂ 8 mg/ml, sodium hyaluronate 2 mg/ml and stirring time of 60 min) completely prevented the growth of Staphylococcus aureus and Escherichia coli. It can be concluded that sodium hyaluronate-TiO₂ bionanocomposite can be used as an effective antimicrobial compound in food, pharmaceutical, medical and environmental sectors.