Overexpression and purification of an immunologically reactive His–BIV capsid fusion protein
D Betemps, F Mallet, V Cheynet, T Baron - Protein expression and …, 1999 - Elsevier
The gene of the capsid protein of bovine immunodeficiency virus (BIV) was linked to a
sequence encoding for six histidines and expressed as the (His) 6p26 capsid fusion protein.
The fusion protein was strongly expressed as both soluble and insoluble forms after
induction by isopropylthio-β-d-galactoside. Purification was based on interaction of the hexa-
histidine polypeptide with metal ions. Expression could represent 11% of the total protein
inEscherichia coli, allowing more than 20 mg of highly purified protein to be obtained per …
sequence encoding for six histidines and expressed as the (His) 6p26 capsid fusion protein.
The fusion protein was strongly expressed as both soluble and insoluble forms after
induction by isopropylthio-β-d-galactoside. Purification was based on interaction of the hexa-
histidine polypeptide with metal ions. Expression could represent 11% of the total protein
inEscherichia coli, allowing more than 20 mg of highly purified protein to be obtained per …
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