Penetrating capacity of human spermatozoa cool preserved in electrolyte-free solution.
S Quan, S Yamano, K Nakagawa, M Irahara… - The Journal of …, 2001 - europepmc.org
S Quan, S Yamano, K Nakagawa, M Irahara, M Kamada, T Aono
The Journal of Reproductive Medicine, 2001•europepmc.orgObjective To evaluate whether human spermatozoa preserved in electrolyte-free (EF)
solution at 4 degrees C possess normal penetrating capacity. Study design The acrosomal
status of human spermatozoa cool preserved in EF solution was evaluated before
preservation and before and after reinitiation by using chlortetracycline staining. The zona-
free hamster egg sperm penetration test was performed using spermatozoa cool preserved
in EF solution. Results The percentages of capacitated and acrosome-reacted spermatozoa …
solution at 4 degrees C possess normal penetrating capacity. Study design The acrosomal
status of human spermatozoa cool preserved in EF solution was evaluated before
preservation and before and after reinitiation by using chlortetracycline staining. The zona-
free hamster egg sperm penetration test was performed using spermatozoa cool preserved
in EF solution. Results The percentages of capacitated and acrosome-reacted spermatozoa …
Objective
To evaluate whether human spermatozoa preserved in electrolyte-free (EF) solution at 4 degrees C possess normal penetrating capacity.
Study design
The acrosomal status of human spermatozoa cool preserved in EF solution was evaluated before preservation and before and after reinitiation by using chlortetracycline staining. The zona-free hamster egg sperm penetration test was performed using spermatozoa cool preserved in EF solution.
Results
The percentages of capacitated and acrosome-reacted spermatozoa cool preserved in EF solution before reinitiation were similar to those of fresh spermatozoa, but they significantly increased after reinitiation. The penetration rate and fertility index of spermatozoa cool preserved in EF solution were comparable to those of fresh spermatozoa (48.3% vs. 50.8% and 1.37+/-0.15 vs. 1.29+/-0.10, respectively).
Conclusion
Human spermatozoa cool preserved in EF solution for one week can possess as much penetrating capacity as fresh spermatozoa.
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