Preparation of fucoidan-chitosan hydrogel and its application as burn healing accelerator on rabbits
Biological and Pharmaceutical Bulletin, 2008•jstage.jst.go.jp
MATERIALS AND METHODS Materials Chitosan (MW 250 kDa, deacetylation degree 90%,
Pronova A/S, Norway; MW 400kDa, deacetylation degree 60%, Fluka, Germany; MW 750
kDa, deacetylation degree 75%, Sigma, USA), Fucoidan (from Fucus vesiculosus) and lactic
acid (85% m/v) purchased from Sigma, USA All other reagents used were of analytical
grade. Preparation of Hydrogels The composition of gel formulations was given in Table 1.
Fucoidan was dissolved in 1% m/v lactic acid solution by mechanical shaking at 300rpm for …
Pronova A/S, Norway; MW 400kDa, deacetylation degree 60%, Fluka, Germany; MW 750
kDa, deacetylation degree 75%, Sigma, USA), Fucoidan (from Fucus vesiculosus) and lactic
acid (85% m/v) purchased from Sigma, USA All other reagents used were of analytical
grade. Preparation of Hydrogels The composition of gel formulations was given in Table 1.
Fucoidan was dissolved in 1% m/v lactic acid solution by mechanical shaking at 300rpm for …
MATERIALS AND METHODS
Materials Chitosan (MW 250 kDa, deacetylation degree 90%, Pronova A/S, Norway; MW 400kDa, deacetylation degree 60%, Fluka, Germany; MW 750 kDa, deacetylation degree 75%, Sigma, USA), Fucoidan (from Fucus vesiculosus) and lactic acid (85% m/v) purchased from Sigma, USA All other reagents used were of analytical grade.
Preparation of Hydrogels The composition of gel formulations was given in Table 1. Fucoidan was dissolved in 1% m/v lactic acid solution by mechanical shaking at 300rpm for 1h. Then chitosan was left to swell in the fucoidan solution overnight to prepare hydrogels. 13) Formulations were kept at 4 C until experiment. Viscosity Measurement The viscosity of hydrogels was determined by using a rotational viscosimeter (Brookfield DV-II, USA) at 25 C. The results were expressed as a mean of three measurements.
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