Quantitative analysis of Anaplasma marginale acquisition and transmission by Dermacentor andersoni fed in vitro

R Vimonish, WC Johnson, MR Mousel, KA Brayton… - Scientific reports, 2020 - nature.com
In this study, we describe a new in vitro tick feeding system that facilitates the study of ticks
and tick-borne pathogens. To optimize the system, we used Dermacentor andersoni and
Anaplasma marginale as a tick-pathogen interaction model. Ticks were fed on bovine blood
containing 10-fold dilutions of the pathogen to determine the effect of dose on tick infection
rate. After feeding on infected blood, ticks were transferred to uninfected blood to stimulate
bacterial replication within the tick vector. During stimulation feeding, blood samples were …

[引用][C] Analysis of Anaplasma marginale acquisition and transmission by Dermacentor andersoni fed in vitro

V Rubikah, MM Ranae, NS Marite - 2020
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