Spectrum of fatty acids synthesized in situ and metabolic heterogeneity of free fatty acids and glycerides within isolated rat adipocytes
J Winand, J Furnelle, C Wodon, J Christophe - Biochimica et Biophysica …, 1971 - Elsevier
1. Lipid metabolism was explored in isolated rat adipose cells incubated in the presence of
10 mM glucose and a low concentration (0.05–0.2 mM) of a labelled pre-cursor ([1-14 C]-
cetate or [1-14 C] palmitate). Six lipid classes were partitioned by thin-layer chromatography
and their fatty acids were analyzed by radio gas-liquid chromatography. 2. 2. Since the
specific activity of free fatty acids was always lower intracellularly than in the medium, it
follows that the intracellular pool of free fatty acids was heterogeneous. Free fatty acids …
10 mM glucose and a low concentration (0.05–0.2 mM) of a labelled pre-cursor ([1-14 C]-
cetate or [1-14 C] palmitate). Six lipid classes were partitioned by thin-layer chromatography
and their fatty acids were analyzed by radio gas-liquid chromatography. 2. 2. Since the
specific activity of free fatty acids was always lower intracellularly than in the medium, it
follows that the intracellular pool of free fatty acids was heterogeneous. Free fatty acids …
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