The Aspergillus PacC zinc finger transcription factor mediates regulation of both acid‐and alkaline‐expressed genes by ambient pH.
J Tilburn, S Sarkar, DA Widdick, EA Espeso… - The EMBO …, 1995 - embopress.org
J Tilburn, S Sarkar, DA Widdick, EA Espeso, M Orejas, J Mungroo, MA Penalva, HN Arst Jr
The EMBO Journal, 1995•embopress.orgThe pH regulation of gene expression in Aspergillus nidulans is mediated by pacC, whose
678 residue‐derived protein contains three putative Cys2His2 zinc fingers. Ten pacCc
mutations mimicking growth at alkaline pH remove between 100 and 214 C‐terminal
residues, including a highly acidic region containing an acidic glutamine repeat. Nine
pacC+/‐mutations mimicking acidic growth conditions remove between 299 and 505 C‐
terminal residues. Deletion of the entire pacC coding region mimics acidity but leads …
678 residue‐derived protein contains three putative Cys2His2 zinc fingers. Ten pacCc
mutations mimicking growth at alkaline pH remove between 100 and 214 C‐terminal
residues, including a highly acidic region containing an acidic glutamine repeat. Nine
pacC+/‐mutations mimicking acidic growth conditions remove between 299 and 505 C‐
terminal residues. Deletion of the entire pacC coding region mimics acidity but leads …
The pH regulation of gene expression in Aspergillus nidulans is mediated by pacC, whose 678 residue‐derived protein contains three putative Cys2His2 zinc fingers. Ten pacCc mutations mimicking growth at alkaline pH remove between 100 and 214 C‐terminal residues, including a highly acidic region containing an acidic glutamine repeat. Nine pacC+/‐ mutations mimicking acidic growth conditions remove between 299 and 505 C‐terminal residues. Deletion of the entire pacC coding region mimics acidity but leads additionally to poor growth and conidiation. A PacC fusion protein binds DNA with the core consensus GCCARG. At alkaline ambient pH, PacC activates transcription of alkaline‐expressed genes (including pacC itself) and represses transcription of acid‐expressed genes. pacCc mutations obviate the need for pH signal transduction.
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