The capsaicin receptor participates in artificial sweetener aversion

CE Riera, H Vogel, SA Simon, S Damak… - … and biophysical research …, 2008 - Elsevier
CE Riera, H Vogel, SA Simon, S Damak, J le Coutre
Biochemical and biophysical research communications, 2008Elsevier
Artificial sweeteners such as saccharin, aspartame, acesulfame-K, and cyclamate produce
at high concentrations an unpleasant after-taste that is generally attributed to bitter and
metallic taste sensations. To identify receptors involved with the complex perception of the
above compounds, preference tests were performed in wild-type mice and mice lacking the
TRPV1 channel or the T1R3 receptor, the latter being necessary for the perception of sweet
taste. The sweeteners, including cyclamate, displayed a biphasic response profile, with the …
Artificial sweeteners such as saccharin, aspartame, acesulfame-K, and cyclamate produce at high concentrations an unpleasant after-taste that is generally attributed to bitter and metallic taste sensations. To identify receptors involved with the complex perception of the above compounds, preference tests were performed in wild-type mice and mice lacking the TRPV1 channel or the T1R3 receptor, the latter being necessary for the perception of sweet taste. The sweeteners, including cyclamate, displayed a biphasic response profile, with the T1R3 mediated component implicated in preference. At high concentrations imparting off-taste, omission of TRPV1 reduced aversion. In a heterologous expression system the Y511A point mutation in the vanilloid pocket of TRPV1 did not affect saccharin and aspartame responses but abolished cyclamate and acesulfame-K activities. The results rationalize artificial sweetener tastes and off-tastes by showing that at low concentrations, these molecules stimulate the gustatory system through the hedonically positive T1R3 pathway, and at higher concentrations, their aversion is partly mediated by TRPV1.
Elsevier
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