The inhibition of the potassium channel TASK-1 in rat cardiac muscle by endothelin-1 is mediated by phospholipase C
J Schiekel, M Lindner, A Hetzel… - Cardiovascular …, 2013 - academic.oup.com
Cardiovascular research, 2013•academic.oup.com
Aims The two-pore-domain potassium channel TASK-1 is robustly inhibited by the activation
of receptors coupled to the Gαq subgroup of G-proteins, but the signal transduction pathway
is still unclear. We have studied the mechanisms by which endothelin receptors inhibit the
current carried by TASK-1 channels (I TASK) in cardiomyocytes. Methods and results Patch-
clamp measurements were carried out in isolated rat cardiomyocytes. I TASK was identified
by extracellular acidification to pH 6.0 and by the application of the TASK-1 blockers A293 …
of receptors coupled to the Gαq subgroup of G-proteins, but the signal transduction pathway
is still unclear. We have studied the mechanisms by which endothelin receptors inhibit the
current carried by TASK-1 channels (I TASK) in cardiomyocytes. Methods and results Patch-
clamp measurements were carried out in isolated rat cardiomyocytes. I TASK was identified
by extracellular acidification to pH 6.0 and by the application of the TASK-1 blockers A293 …
Aims
The two-pore-domain potassium channel TASK-1 is robustly inhibited by the activation of receptors coupled to the Gαq subgroup of G-proteins, but the signal transduction pathway is still unclear. We have studied the mechanisms by which endothelin receptors inhibit the current carried by TASK-1 channels (ITASK) in cardiomyocytes.
Methods and results
Patch-clamp measurements were carried out in isolated rat cardiomyocytes. ITASK was identified by extracellular acidification to pH 6.0 and by the application of the TASK-1 blockers A293 and A1899. Endothelin-1 completely inhibited ITASK with an EC50 of <10 nM; this effect was mainly mediated by endothelin-A receptors. Application of 20 nM endothelin-1 caused a significant increase in action potential duration under control conditions; this was significantly reduced after pre-incubation of the cardiomyocytes with 200 nM A1899. The inhibition of ITASK by endothelin-1 was not affected by inhibitors of protein kinase C or rho kinase, but was strongly reduced by U73122, an inhibitor of phospholipase C (PLC). The ability of endothelin-1 to activate PLC-mediated signalling pathways was examined in mammalian cells transfected with TASK-1 and the endothelin-A receptor using patch-clamp measurements and total internal reflection microscopy. U73122 prevented the inhibition of ITASK by endothelin-1 and blocked PLC-mediated signalling, as verified with a fluorescent probe for phosphatidylinositol-(4,5)-bisphosphate hydrolysis.
Conclusion
Our results show that ITASK in rat cardiomyocytes is controlled by endothelin-1 and suggest that the inhibition of TASK-1 via endothelin receptors is mediated by the activation of PLC. The prolongation of the action potential observed with 20 nM endothelin-1 was mainly due to the inhibition of ITASK.
Oxford University Press
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