The role of phosphocreatine kinase in the motility of human spermatozoa supported by different metabolic substrates

CH Yeung, GC Majumder, C Rolf… - MHR: Basic science …, 1996 - academic.oup.com
In the spermatozoa of some species creatine kinase (CK: EC 2.7. 3.2) is involved in shuttling
energy, in the form of creatine phosphate, between the mid-piece mitochondria and
flagellum. In this study, the effects of the CK inhibitor dinitrofluorobenzene (DNFB) on human
sperm CK activity, motility and ATP concentrations were assessed with different energy
substrates. There was a dose-dependent inhibition of CK activity by DNFB but inhibition was
incomplete and there was no effect on the percentage of flagellating cells, irrespective of …
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