The small variant of the apoptosis-associated X-chromosome RBM10 gene is co-expressed with caspase-3 in breast cancer
E Martin-Garabato, F Martinez-Arribas… - Cancer Genomics & …, 2008 - cgp.iiarjournals.org
E Martin-Garabato, F Martinez-Arribas, M Pollan, AR Lucas, J Sanchez, J Schneider
Cancer Genomics & Proteomics, 2008•cgp.iiarjournals.orgBackground: There are very few studies on the final triggers of apoptosis, the caspases, in
breast cancer. Materials and Methods: Caspase-3 expression was studied by means of
reverse transcript polymerase chain reaction (RT-PCR) in a series of 108 previously
untreated patients with breast cancer. Expression levels were correlated with those obtained
using the same technique of the apoptosis-associated X-chromosome genes RBMX, RBM3,
RBM10 small and RBM10 large variant; Bcl-2 and Bax; the angiogenesis-associated genes …
breast cancer. Materials and Methods: Caspase-3 expression was studied by means of
reverse transcript polymerase chain reaction (RT-PCR) in a series of 108 previously
untreated patients with breast cancer. Expression levels were correlated with those obtained
using the same technique of the apoptosis-associated X-chromosome genes RBMX, RBM3,
RBM10 small and RBM10 large variant; Bcl-2 and Bax; the angiogenesis-associated genes …
Background
There are very few studies on the final triggers of apoptosis, the caspases, in breast cancer. Materials and Methods
Caspase-3 expression was studied by means of reverse transcript polymerase chain reaction (RT-PCR) in a series of 108 previously untreated patients with breast cancer. Expression levels were correlated with those obtained using the same technique of the apoptosis-associated X-chromosome genes RBMX, RBM3, RBM10 small and RBM10 large variant; Bcl-2 and Bax; the angiogenesis-associated genes VEGF and CD105 (endoglin); hMAM and Nup88. The correlation with the expression of hormone receptors, c-erb-B2, mutant p53 and Ki-67, all measured by means of immunohistochemistry, was also studied, as well as that with standard clinical parameters such as histological type, tumor size, axillary metastasis and DNA-ploidy. Results
The only statistically significant correlations observed between caspase-3 mRNA expression and the parameters tested were a direct one with both the Bax (p=0.007) and the small variant of the X-chromosome RBM10 gene (p=0.018), and an inverse one with the angiogenesis-associated CD105 (endoglin) gene (p=0.044). Conclusion
These results indicate that very few genes are involved in the last steps of the apoptotic cascade in breast cancer, among them one of the X-chromosome RBM family. They also support the relatively unexplored link between apoptosis and angiogenesis.cgp.iiarjournals.org
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