The synthesis of influenza virus negative-strand RNA takes place in insoluble complexes present in the nuclear matrix fraction

JA López-Turiso, C Martínez, T Toshiki, J Ortín - Virus research, 1990 - Elsevier
The replication of influenza virus RNA in vitro has been studied by cell fractionation of
MDCK-infected cells and characterization of in vitro synthesized RNA. Analysis of the RNA
product polarity by liquid hybridization to excess single-stranded DNA probes shows that
only the RNP complexes present in the nuclear matrix fraction are able to synthesize
negative-polarity RNA. This RNA product has been characterized as authentic vRNA by size
analysis, RNase-protection by unlabelled, positive-polarity riboprobes and T1-finterprinting …
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