Use of TrpE fusion protein to identify antigenic domains within the BIV envelope protein
P Chen, ZQ Liu, C Wood - Journal of virological methods, 1994 - Elsevier
Nine different recombinant clones spanning various regions of the bovine immunodeficiency-
like virus (BIV) envelope gene open reading frame were generated. These clones span the
entire external glycoprotein as well as the transmembrane glycoprotein region. These
proteins were expressed as fusions to the TrpE protein in E. coli. The levels of recombinant
protein expressed varied, some clones expressed enough protein that can be detected in a
Coomassie blue-stained gel, whereas other proteins could only be detected by Western blot …
like virus (BIV) envelope gene open reading frame were generated. These clones span the
entire external glycoprotein as well as the transmembrane glycoprotein region. These
proteins were expressed as fusions to the TrpE protein in E. coli. The levels of recombinant
protein expressed varied, some clones expressed enough protein that can be detected in a
Coomassie blue-stained gel, whereas other proteins could only be detected by Western blot …
Use of TrpE fusion protein to identify antigenic domains within the BIV [bovine immunodeficiency virus] envelope protein.
P Chen, ZQ Lui, C Wood - 1994 - cabidigitallibrary.org
Nine different recombinant clones spanning various regions of the bovine immunodeficiency-
like virus (BIV) envelope gene open reading frame were generated. These clones span the
entire external glycoprotein and the transmembrane glycoprotein region. These proteins
were expressed as fusions to the TrpE protein in Escherichia coli. The levels of recombinant
protein expressed varied, some clones expressed enough protein to be detectable in a
Coomassie blue-stained gel; other could only be detected by Western blot analyses. A …
like virus (BIV) envelope gene open reading frame were generated. These clones span the
entire external glycoprotein and the transmembrane glycoprotein region. These proteins
were expressed as fusions to the TrpE protein in Escherichia coli. The levels of recombinant
protein expressed varied, some clones expressed enough protein to be detectable in a
Coomassie blue-stained gel; other could only be detected by Western blot analyses. A …
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