Utilization of fadA knockout mutant Pseudomonas putida for overproduction of medium chain-length-polyhydroxyalkanoate

MT Vo, KW Lee, TK Kim, YH Lee - Biotechnology letters, 2007 - Springer
The fadBA operon in the fatty acid β-oxidation pathway of P. putida KCTC1639 was blocked
to induce a metabolic flux of the intermediates to the biosynthesis of medium chain-length
PHA (mcl-PHA). Succinate at 150 mg l− 1 stimulated cell growth and also the biosynthesis of
medium chain-length-polyhydroxyalkanoate. pH-stat fed-batch cultivation of the fadA
knockout mutant P. putida KCTC1639 was carried out for 60 h, in which mcl-PHA reached 8
gl− 1 with a cell dry weight of 10.3 gl− 1.

Utilization of fadA knockout mutant Pseudomonas putida for overproduction of medium chain-length-polyhydroxyalkanoate.

MTV Minh Tri Vo, LKW Lee KwangWoo… - 2007 - cabidigitallibrary.org
The fadBA operon in the fatty acid β-oxidation pathway of P. putida KCTC1639 was blocked
to induce a metabolic flux of the intermediates to the biosynthesis of medium chain-length
PHA (mcl-PHA). Succinate at 150 mg l-1 stimulated cell growth and also the biosynthesis of
medium chain-length-polyhydroxyalkanoate. pH-stat fed-batch cultivation of the fadA
knockout mutant P. putida KCTC1639 was carried out for 60 h, in which mcl-PHA reached 8
g l-1 with a cell dry weight of 10.3 g l-1.
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