[引用][C] Valproate-Induced Alterations of Coenzyme A and Coenzyme A Ester Concentrations in Human Kidney Tubules Metabolizing Glutamine1
D Durozard, G Martin, G Baverel - Molecular aspects of …, 1991 - karger.com
D Durozard, G Martin, G Baverel
Molecular aspects of ammoniagenesis, 1991•karger.comMaterials and Methods Fresh human kidney cortex was obtained from the uninvolved pole of
kidneys removed for neoplasm from 18-hour-fasted adult patients. Specimens of cortex were
immediately dissected and placed in ice-cold Krebs-Henseleit buffer (pH 7.4) gassed with a
mixture of 02/C02 (19: 1) until the beginning of the tubule isolation period (usually within 10
min).
kidneys removed for neoplasm from 18-hour-fasted adult patients. Specimens of cortex were
immediately dissected and placed in ice-cold Krebs-Henseleit buffer (pH 7.4) gassed with a
mixture of 02/C02 (19: 1) until the beginning of the tubule isolation period (usually within 10
min).
Materials and Methods
Fresh human kidney cortex was obtained from the uninvolved pole of kidneys removed for neoplasm from 18-hour-fasted adult patients. Specimens of cortex were immediately dissected and placed in ice-cold Krebs-Henseleit buffer (pH 7.4) gassed with a mixture of 02/C02 (19: 1) until the beginning of the tubule isolation period (usually within 10 min).
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