[HTML][HTML] In vitro characterization of dental pulp stem cells cultured in two microsphere-forming culture plates

NU Bu, HS Lee, BN Lee, YC Hwang, SY Kim… - Journal of Clinical …, 2020 - mdpi.com
Various three-dimensional (3D) culture methods have been introduced to overcome the
limitations of in vitro culture and mimic in vivo conditions. This study aimed to evaluate two …

A comprehensive analysis of human dental pulp cell spheroids in a three-dimensional pellet culture system

S Zhang, P Buttler-Buecher, B Denecke… - Archives of oral …, 2018 - Elsevier
Abstract Objective Three-dimensional (3D) cell culture methods are of high importance to
studies of biological processes. This is particularly the case with spheroid cultures, which …

Three-dimensional sphere-forming cells are unique multipotent cell population in dental pulp cells

SH Lee, A Inaba, N Mohindroo, D Ganesh… - Journal of …, 2017 - Elsevier
Abstract Introduction Mesenchymal stem cells (MSCs) are typically cultured as adherent
monolayer using a conventional tissue culture technique. However, this technique …

Cell‐derived micro‐environment helps dental pulp stem cells promote dental pulp regeneration

X Zhang, H Li, J Sun, X Luo, H Yang, L Xie… - Cell …, 2017 - Wiley Online Library
Objectives The function of the dental pulp is closely connected to the extracellular matrix
(ECM) structure, and ECM has received significant attention due to its biological functions for …

[HTML][HTML] The establishment of a chemically defined serum-free culture system for human dental pulp stem cells

J Xiao, D Yang, Q Li, W Tian, W Guo - Stem cell research & therapy, 2018 - Springer
Background The concept of establishing a dental stem cell (DSC) bank for oral and
maxillofacial regeneration has become of great interest but it remains at a primitive stage …

Phenotypic and functional comparison of optimum culture conditions for upscaling of dental pulp stem cells

MM Kanafi, R Pal, PK Gupta - Cell biology international, 2013 - Wiley Online Library
Advances in dental pulp stem cell (DPSC) biology and behaviour have promised much in
the field of regenerative medicine. Their recent use in clinical trials for bone repair enforces …

Properties of dental pulp–derived mesenchymal stem cells and the effects of culture conditions

N Kawashima, S Noda, M Yamamoto, T Okiji - Journal of Endodontics, 2017 - Elsevier
Dental pulp mesenchymal stem cells (DPMSCs) highly express mesenchymal stem cell
markers and possess the potential to differentiate into neural cells, osteoblasts, adipocytes …

Three-dimensional Spheroid Culture Enhances Multipotent Differentiation and Stemness Capacities of Human Dental Pulp‐derived Mesenchymal Stem Cells by …

YH Chan, YC Lee, CY Hung, PJ Yang, PC Lai… - Stem Cell Reviews and …, 2021 - Springer
Abstract Background Three-dimensional (3D) culture of mesenchymal stem cells has
become an important research and development topic. However, comprehensive analysis of …

[HTML][HTML] Effect of cell culture density on dental pulp-derived mesenchymal stem cells with reference to osteogenic differentiation

S Noda, N Kawashima, M Yamamoto, K Hashimoto… - Scientific Reports, 2019 - nature.com
Dental pulp stem cells (DPSCs) are a good source for tissue regeneration, however, the
number of DPSCs in the pulp tissue is limited. Cell propagation is essential for tissue …

[HTML][HTML] Single-cell characterization of monolayer cultured human dental pulp stem cells with enhanced differentiation capacity

Y Cui, W Ji, Y Gao, Y Xiao, H Liu, Z Chen - International Journal of Oral …, 2021 - nature.com
Human dental pulp stem cells (hDPSCs) are easily obtained multipotent cells, however, their
potential value in regenerative medicine is hindered by the phenotypic and functional …