Single-cell RNA-seq (scRNA-seq) provides a comprehensive measurement of stochasticity
in transcription, but the limitations of the technology have prevented its application to dissect …

The emergence of single-cell RNA-seq (scRNA-seq) technology has made it possible to
measure gene expression variations at cellular level. This breakthrough enables the …

Background Early single-cell RNA-seq (scRNA-seq) studies suggested that it was unusual
to see more than one isoform being produced from a gene in a single cell, even when …

RNA splicing is an important driver of heterogeneity in single cells through the expression of
alternative transcripts and as a determinant of transcriptional kinetics. However, the intrinsic …

Single-cell RNA-seq mammalian transcriptome studies are at an early stage in uncovering
cell-to-cell variation in gene expression, transcript processing and editing, and regulatory …

Motivation: In eukaryotic cells, alternative splicing expands the diversity of RNA transcripts
and plays an important role in tissue-specific differentiation, and can be misregulated in …

Single cell RNA-seq experiments provide valuable insight into cellular heterogeneity but
suffer from low coverage, 3′ bias and technical noise. These unique properties of single …

Single-cell RNAseq and alternative splicing studies have recently become two of the most
prominent applications of RNAseq. However, the combination of both is still challenging …

Single-cell RNA sequencing provides powerful insight into the factors that determine each
cell's unique identity. Previous studies led to the surprising observation that alternative …

Motivation: Alternative splicing (AS) is a pre-mRNA maturation process leading to the
expression of multiple mRNA variants from the same primary transcript. More than 90% of …