The Ca2+-triggered luciferin-binding protein of Renilla reniformis (RLBP) is a non-covalent
complex of apoprotein (apoRLBP) and coelenterazine (luciferin). The gene encoding …

A Ca2+-triggered luciferin-binding protein (BP-LH2) from the bioluminescent marine
coelenterate, Renilla reniformis, has been purified by conventional methods. One kilogram …

The Renilla bioluminescent system in vivo is comprised of three proteins—the luciferase,
green-fluorescent protein, and coelenterazine-binding protein (CBP), previously called …

The complete amino acid sequence of the Ca 2+-triggered luciferin binding protein (LBP) of
Renilla reniformis has been determined. The apoprotein has an unblocked amino terminus …

Renilla reniformis is an anthozoan coelenterate capable of exhibiting bioluminescence.
Bioluminescence in Renilla results from the oxidation of coelenterate luciferin …

Ca2+-triggered coelenterazine-binding protein (CBP) is a natural form of the luciferase
substrate involved in the Renilla bioluminescence reaction. It is a stable complex of …

We expressed luciferase (RLuc) from Renilla reniformis in Escherichia coli. RLuc was
purified using a Ni-NTA column and subsequently characterized. It was unstable in acidic …

A cDNA encoding the Renilla reniformis luciferase was expressed in simian and murine
cells in a transient and stable manner, respectively. Light emission catalyzed by luciferase …

The recombinant coelenterazine-dependent luciferases (isoforms MLuc164 and MLuc39)
from the marine copepod Metridia longa were expressed asinclusion bodies in E. coli cells …

Materials and Methods Materials. All reagents used for synthesis and purification were the
best commercial grades available. Luciferase was prepared as previously described …