ABSTRACT R388 conjugative relaxase TrwC acts as a site-specific recombinase, promoting
recombination between two cognate oriT s on double-stranded DNA substrates. The …

We show that relaxase TrwC promotes recombination between two directly repeated oriTs
while related relaxases TraI of F and pKM101 do not. Efficient recombination required also …

Conjugative relaxases are the proteins that initiate bacterial conjugation by a site-specific
cleavage of the transferred DNA strand. In vitro, they show strand-transferase activity on …

Background Bacterial conjugation is a mechanism for horizontal DNA transfer between
bacteria which requires cell to cell contact, usually mediated by self-transmissible plasmids …

Relaxases are DNA strand transferases that catalyze the initial and final stages of DNA
processing during conjugative cell-to-cell DNA transfer. Upon binding to the origin of transfer …

Bacterial conjugation is a mechanism of horizontal DNA transfer. The relaxase TrwC of the
conjugative plasmid R388 cleaves one strand of the transferred DNA at the oriT gene …

Relaxosomes are specific nucleoprotein structures involved in DNA‐processing reactions
during bacterial conjugation. In this work, we present evidence indicating that plasmid R388 …

The transfer of DNA across membranes and between cells is a central biological process;
however, its molecular mechanism remains unknown. In prokaryotes, trans-membrane …

TrwC is a relaxase protein, which starts and finishes DNA processing during bacterial
conjugation in plasmid R388. TrwC recognizes a specific sequence of DNA (25 nucleotides) …

Protein TrwC is the conjugative relaxase responsible for DNA processing in plasmid R388
bacterial conjugation. TrwC has two catalytic tyrosines, Y18 and Y26, both able to carry out …