We have used random sequence mutagenesis to generate mutants of DNA polymerase β in
an effort to identify amino acid residues important for function, catalytic efficiency and fidelity …

DNA polymerase β (pol β) is a 39-kDa protein that functions in DNA repair processes in
mammalian cells. As a first step toward understanding mechanisms of polymerase fidelity …

Materials and Methods Site-Directed Mutagenesis. Expression plasmid JMp/35, which
contained the whole coding sequence for rat DNA polymerase/3 in M13 phage-plasmid …

We used quantitative complementation assays to characterize individual DNA polymerase β
(Pol β) mutants for their ability to function in DNA replication and DNA repair. We also …

We developed an in vivo selection to identify 3′-azido-3′-deoxythymidine (AZT)-resistant
mutants of rat DNA polymerase β (pol β). The selection utilizes pol β's ability to substitute for …

The frequency and specificity of mutations produced during in vitro DNA synthesis of the
lacZ alpha gene in M13mp2 DNA by eucaryotic DNA polymerase-alpha (pol-alpha) and …

Publisher Summary This chapter discusses the rationale for mutagenesis of DNA
polymerase active sites. Site-directed mutations were introduced to each of the highly …

DNA polymerase β (pol β) from rat brain, overexpressed in Escherichia coli, was used as a
model to study the factors responsible for substrate specificity [k pol, K d (app) and k pol/K d …

The catalytic polypeptide of human DNA polymerase δ was overexpressed in BSC-40 cells
(African green monkey kidney cell line) using the vaccinia virus/pTM1 system. The …

Primary structure of the catalytic subunit of calf thymus DNA polymerase .delta.: sequence
similarities with other DNA polymeras Page 1 11742 Biochemistry 1991, 30, 11742-11750 …