Transmission electron microscopy of zebrafish spinal motor nerve roots

AD Morris, A Erisir, SJ Criswell… - Developmental …, 2017 - Wiley Online Library
Background: Spinal motor nerves are essential for relaying information between the central
and peripheral nervous systems. Perturbations to cell types that comprise these nerves may …

Zebrafish in Situ spinal cord preparation for electrophysiological recordings from spinal sensory and Motor Neurons

RL Moreno, M Josey, AB Ribera - JoVE (Journal of Visualized …, 2017 - jove.com
Zebrafish, first introduced as a developmental model, have gained popularity in many other
fields. The ease of rearing large numbers of rapidly developing organisms, combined with …

Volume Electron Microscopic Analyses in the Larval Zebrafish

FN Svara - 2017 - archiv.ub.uni-heidelberg.de
The goal of this work was two-fold: 1) To apply serial block-face electron microscopy (SBEM)
to the spinal cord of a larval zebrafish, in order to gain a mechanistic understanding of …

Primary cell culture of adult zebrafish spinal neurons for electrophysiological studies

ME Meade, JE Roginsky, JR Schulz - Journal of neuroscience methods, 2019 - Elsevier
Abstract Background Zebrafish (Danio rerio) are growing in popularity as a vertebrate model
organism for the study of spinal neurocircuitry and locomotion. While many studies have …

Transmission electron microscopy for zebrafish larvae and adult lateral line nerve

RL Cunningham, KR Monk - Schwann Cells: Methods and Protocols, 2018 - Springer
Transmission electron microscopy (TEM) enables visualization of the ultrastructure of the
myelin sheath. Schwann cells on the posterior lateral line nerves and motor nerves can be …

Locomotor pattern in the adult zebrafish spinal cord in vitro

JP Gabriel, R Mahmood, AM Walter… - Journal of …, 2008 - journals.physiology.org
The zebrafish is an attractive model system for studying the function of the spinal locomotor
network by combining electrophysiological, imaging, and genetic approaches. Thus far …

[HTML][HTML] Protocol to visualize distinct motoneuron pools in adult zebrafish via injection of retrograde tracers

M Bertuzzi, I Pallucchi, A El Manira - STAR protocols, 2022 - Elsevier
In adult zebrafish, slow, intermediate, and fast muscle fibers occupy distinct regions of the
axial muscle, allowing the use of retrograde tracers for selective targeting of the …

Development of spinal neurons and tracts in the zebrafish embryo

JY Kuwada, RR Bernhardt… - Journal of comparative …, 1990 - Wiley Online Library
We have analyzed pathfinding by growth cones in the spinal cord of the early zebrafish
embryo, because it is an extremely simple system. At 18–20 hours of development the spinal …

Dissection and lateral mounting of zebrafish embryos: analysis of spinal cord development

AP Beck, RM Watt, J Bonner - JoVE (Journal of Visualized Experiments), 2014 - jove.com
The zebrafish spinal cord is an effective investigative model for nervous system research for
several reasons. First, genetic, transgenic and gene knockdown approaches can be utilized …

Neuronal labeling patterns in the spinal cord of adult transgenic Zebrafish

A Stil, P Drapeau - Developmental Neurobiology, 2016 - Wiley Online Library
We describe neuronal patterns in the spinal cord of adult zebrafish. We studied the
distribution of cells and processes in the three spinal regions reported in the literature: the …