[PDF][PDF] A second divalent metal ion in the group II intron reaction center
PM Gordon, R Fong, JA Piccirilli - Chemistry & biology, 2007 - cell.com
Group II introns are mobile genetic elements that have been implicated as agents of genetic
diversity, and serve as important model systems for investigating RNA catalysis and pre …
diversity, and serve as important model systems for investigating RNA catalysis and pre …
Structural requirement for Mg2+ binding in the group I intron core
P Rangan, SA Woodson - Journal of molecular biology, 2003 - Elsevier
Divalent metal ions are required for splicing of group I introns, but their role in maintaining
the structure of the active site is still under investigation. Ribonuclease and hydroxyl radical …
the structure of the active site is still under investigation. Ribonuclease and hydroxyl radical …
Unexpected metal ion requirements specific for catalysis of the branching reaction in a group II intron
E Dème, A Nolte, A Jacquier - Biochemistry, 1999 - ACS Publications
The splicing process catalyzed by group II intron ribozymes follows the same two-step
pathway as nuclear pre-mRNA splicing. In vivo, the first splicing step of wild-type introns is a …
pathway as nuclear pre-mRNA splicing. In vivo, the first splicing step of wild-type introns is a …
The ribozyme core of group II introns: a structure in want of partners
F Michel, M Costa, E Westhof - Trends in biochemical sciences, 2009 - cell.com
Group II introns contain a large ribozyme, which catalyzes self-splicing, and the coding
sequence of a reverse transcriptase, the function of which is to cooperate with the ribozyme …
sequence of a reverse transcriptase, the function of which is to cooperate with the ribozyme …
Metal ion binding sites in a group II intron core
Group II introns are catalytic RNA molecules that require divalent metal ions for folding,
substrate binding, and chemical catalysis. Metal ion binding sites in the group II core have …
substrate binding, and chemical catalysis. Metal ion binding sites in the group II core have …
RNA splicing: group I intron crystal structures reveal the basis of splice site selection and metal ion catalysis
MR Stahley, SA Strobel - Current opinion in structural biology, 2006 - Elsevier
The group I intron has served as a model for RNA catalysis since its discovery 25 years ago.
Four recently determined high-resolution crystal structures complement extensive …
Four recently determined high-resolution crystal structures complement extensive …
Conformation of the Group II intron branch site in solution
JC Schlatterer, SH Crayton… - Journal of the American …, 2006 - ACS Publications
Group II introns are multidomain ribozymes that catalyze their own removal from pre-mRNA.
The nucleophile for the first cleavage step is the 2 'OH of a specific adenosine within domain …
The nucleophile for the first cleavage step is the 2 'OH of a specific adenosine within domain …
Divalent metal ion binding to a conserved wobble pair defining the upstream site of cleavage of group I self-splicing introns
FHT Allain, G Varani - Nucleic acids research, 1995 - academic.oup.com
The upstream site of cleavage of all group I self-splicing introns is identified by an absolutely
conserved U· G base pair. Although a wobble C· A pair can substitute the U· G pair, all other …
conserved U· G base pair. Although a wobble C· A pair can substitute the U· G pair, all other …
Catalytic reaction mechanisms and structural features of group II intron ribozymes
AM Pyle - Catalytic RNA, 1996 - Springer
Splicing of group II introns is essential for the metabolism of many organisms (Michel et al.
1989; Michel and Ferat 1995). These ubiquitous introns play a critical role in the processing …
1989; Michel and Ferat 1995). These ubiquitous introns play a critical role in the processing …
[HTML][HTML] Visualizing group II intron catalysis through the stages of splicing
Group II introns are self-splicing ribozymes that share a reaction mechanism and a common
ancestor with the eukaryotic spliceosome, thereby providing a model system for …
ancestor with the eukaryotic spliceosome, thereby providing a model system for …