Background Heterologous protein production in Escherichia coli often suffers from
bottlenecks such as proteolytic degradation, complex purification procedures and toxicity …

Recombinant proteins are becoming increasingly important for industrial applications, where
Escherichia coli is the most widely used bacterial host for their production. However, the …

The formation of inclusion bodies (IBs) during expression of recombinant therapeutic
proteins using E. coli is a significant hurdle in producing high-quality, safe, and efficacious …

Escherichia coli is used intensively for recombinant protein production, but one key
challenge with recombinant E. coli is the tendency of recombinant proteins to misfold and …

The formation of inclusion bodies (IBs) is considered as an Achilles heel of heterologous
protein expression in bacterial hosts. Wide array of techniques has been developed to …

The ability to efficiently produce hundreds of proteins in parallel is the most basic
requirement of many aspects of proteomics. Overcoming the technical and financial barriers …

The production of recombinant proteins in the microbial host Escherichia coli often results in
the formation of cytoplasmic protein inclusion bodies (IBs). Proteins forming IBs are often …

During heterologous protein production with Escherichia coli, the formation of inclusion
bodies (IBs) is often a major drawback as these aggregated proteins are usually inactive …

Recombinant proteins expressed as bacterial inclusion bodies (IBs) are now receiving
tremendous attention for many diverse applications in the areas of industrial and medical …

Inclusion bodies (IBs) are typically non-functional particles of aggregated proteins. However,
some proteins in fusion with amyloid-like peptides, viral coat proteins, and cellulose binding …