Transient XylR binding to the UAS of the Pseudomonas putida σ54 promoter Pu revealed with high intensity UV footprinting in vivo

M Valls, V de Lorenzo - Nucleic acids research, 2003 - academic.oup.com
The binding of the transcriptional regulator XylR to its cognate upstream activating
sequences (UAS) of the σ54‐dependent promoter Pu of Pseudomonas putida has been …

Recruitment of RNA Polymerase Is a Rate-limiting Step for the Activation of the ς54 Promoter Pu ofPseudomonas putida

M Carmona, V de Lorenzo, G Bertoni - Journal of Biological Chemistry, 1999 - ASBMB
The activity of the ς 54-promoter Pu of Pseudomonas putida was examined in vitro with a
DNA template lacking upstream activating sequences, such that RNA polymerase can be …

In vitro activities of an N-terminal truncated form of XylR, a σ54-dependent transcriptional activator of Pseudomonas putida

J Pérez-Martı́n, V de Lorenzo - Journal of molecular biology, 1996 - Elsevier
A truncated derivative of the XylR protein, which is able to constitutively activate the σ54-
dependentPupromoter of the TOL (toluene biodegradation) plasmid ofPseudomonas putida …

The sigma 54-dependent promoter Ps of the TOL plasmid of Pseudomonas putida requires HU for transcriptional activation in vivo by XylR

J Pérez-Martín, V de Lorenzo - Journal of bacteriology, 1995 - Am Soc Microbiol
In the presence of toluene and xylenes, the sigma 54-dependent Ps promoter of the TOL
(toluene biodegradation) plasmid pWW0 of Pseudomonas putida is activated at a distance …

Integration host factor suppresses promiscuous activation of the sigma 54-dependent promoter Pu of Pseudomonas putida.

J Pérez-Martín, V De Lorenzo - Proceedings of the National …, 1995 - National Acad Sciences
In the presence of m-xylene, the Pu promoter of the TOL plasmid of Pseudomonas putida is
activated by the prokaryotic enhancer-binding protein XylR. The intervening DNA segment …

Identification of the repressor subdomain within the signal reception module of the prokaryotic enhancer-binding protein XylR of Pseudomonas putida

J Pérez-Martín, V de Lorenzo - Journal of Biological Chemistry, 1996 - ASBMB
In the presence of m-xylene, the protein XylR encoded by the TOL plasmid of Pseudomonas
putida, activates the σ 54-dependent promoter Pu. Early activation stages involve the …

Activation of the transcriptional regulator XylR of Pseudomonas putida by release of repression between functional domains

S Fernández, V de Lorenzo… - Molecular …, 1995 - Wiley Online Library
In the presence of toluene, xylenes and other structural analogues, the regulatory protein
XylR, of the family of transcriptional regulators which act in concert with the σ54 factor …

Physical and Functional Analysis of the Prokaryotic Enhancer of the σ54-promoters of the TOL Plasmid ofPseudomonas putida

J Pérez-Martı́n, V de Lorenzo - Journal of molecular biology, 1996 - Elsevier
The physical and the functional organization of the upstreamcis-acting sequence that
controls at a distance the transcriptional activity ofPuandPs, the two σ54-dependent …

Involvement of σ54 in exponential silencing of the Pseudomonas putida TOL plasmid Pu promoter

II Cases, V de Lorenzo… - Molecular microbiology, 1996 - Wiley Online Library
The σ54‐dependent Pu promoter of the TOL plasmid pWWO of Pseudomonas putida
becomes activated by the prokaryotic enhancer‐binding XyIR protein when cells encounter …

Activation of the toluene‐responsive regulator XylR causes a transcriptional switch between σ54 and σ70 promoters at the divergent Pr/Ps region of the TOL plasmid

G Bertoni, S Marqués, V De Lorenzo - Molecular microbiology, 1998 - Wiley Online Library
The mechanism by which XylR, the toluene‐responsive activator of the σ54‐dependent Pu
and Ps promoters of the Pseudomonas TOL plasmid pWW0, downregulates its own σ70 …