Effect of sperm cryopreservation on sperm DNA stability and progeny development in rainbow trout

C Labbe, A Martoriati, A Devaux… - Molecular Reproduction …, 2001 - Wiley Online Library
This study was carried out to test how sperm cryopreservation affected nuclear DNA stability
and whether progeny development was modified when eggs were fertilized with …

Evaluation of DNA damage in rainbow trout (Oncorhynchus mykiss) and gilthead sea bream (Sparus aurata) cryopreserved sperm

E Cabrita, V Robles, L Rebordinos, C Sarasquete… - Cryobiology, 2005 - Elsevier
Cryopreservation causes several types of damage to spermatozoa, such as loss of plasma
membrane integrity and functionality, loss of motility, and ATP content, resulting in decrease …

Fertilization capacity with rainbow trout DNA-damaged sperm and embryo developmental success

S Pérez-Cerezales, S Martínez-Páramo… - …, 2010 - rep.bioscientifica.com
Mammalian spermatozoa undergo a strong selection process along the female tract to
guarantee fertilization by good quality cells, but risks of fertilization with DNA-damaged …

Altered gene transcription and telomere length in trout embryo and larvae obtained with DNA cryodamaged sperm

S Pérez-Cerezales, A Gutiérrez-Adán… - Theriogenology, 2011 - Elsevier
Sperm cryopreservation could entail DNA damage, promoting base oxidization and strand
breaks. In a previous work we showed that trout DNA damaged sperm is able to fertilize …

Potential biomarkers of DNA quality in cryopreserved fish sperm: impact on gene expression and embryonic development

E Figueroa, M Lee‐Estévez, I Valdebenito… - Reviews in …, 2020 - Wiley Online Library
Sperm DNA quality and gene expression are crucial for early embryo development.
Abnormal genomic processes can cause irreversible damage to totipotent cells, thereby …

Effect of extender composition and equilibration time on fertilization ability and enzymatic activity of rainbow trout cryopreserved spermatozoa

I Babiak, J Glogowski, K Goryczko, S Dobosz… - Theriogenology, 2001 - Elsevier
The effects of extender composition and equilibration time on fertilizing ability of
cryopreserved spermatozoa from rainbow trout, Oncorhynchus mykiss, were investigated. In …

Cryopreservation of fish sperm: applications and perspectives

E Cabrita, C Sarasquete… - Journal of Applied …, 2010 - Wiley Online Library
Cryopreservation is of interest not only for fish farming but also for the conservation and
genetic improvement of resources. This technique has been well established in some …

Evaluation of DNA damage in Dicentrarchus labrax sperm following cryopreservation

L Zilli, R Schiavone, V Zonno, C Storelli, S Vilella - Cryobiology, 2003 - Elsevier
In this paper, DNA laddering analysis and single-cell gel electrophoresis (SCGE) or Comet
assay, were used to detect DNA damage in response to a cryopreservation process in sea …

Evaluation of DNA damage as a quality marker for rainbow trout sperm cryopreservation and use of LDL as cryoprotectant

S Pérez-Cerezales, S Martínez-Páramo, J Beirão… - Theriogenology, 2010 - Elsevier
Defining reliable and objective biomarkers of sperm quality is a complex matter, because it
does not rely on a particular characteristic of the milt. Susceptibility to cryopreservation …

Cryobanking as tool for conservation of biodiversity: effect of brown trout sperm cryopreservation on the male genetic potential

S Martínez-Páramo, S Pérez-Cerezales… - Theriogenology, 2009 - Elsevier
Sperm cryobanking could be a good alternative to breeding in captivity in order to preserve
genetic diversity. Sperm from two well-characterized brown trout populations originating …