Current strategies for protein production and purification enabling membrane protein structural biology
Membrane proteins are still heavily under-represented in the protein data bank (PDB),
owing to multiple bottlenecks. The typical low abundance of membrane proteins in their …
owing to multiple bottlenecks. The typical low abundance of membrane proteins in their …
[HTML][HTML] Overcoming the challenges of membrane protein crystallography
EP Carpenter, K Beis, AD Cameron, S Iwata - Current opinion in structural …, 2008 - Elsevier
Membrane protein structural biology is still a largely unconquered area, given that
approximately 25% of all proteins are membrane proteins and yet less than 150 unique …
approximately 25% of all proteins are membrane proteins and yet less than 150 unique …
Membrane protein structural biology–how far can the bugs take us?
Membrane proteins are core components of many essential cellular processes, and high-
resolution structural data is therefore highly sought after. However, owing to the many …
resolution structural data is therefore highly sought after. However, owing to the many …
Membrane protein structural biology: the high throughput challenge
PJ Loll - Journal of structural biology, 2003 - Elsevier
Membrane proteins represent roughly one-third of the proteins encoded in the genome, yet
fewer than 1% of the proteins are of known structure. High-throughput crystallography offers …
fewer than 1% of the proteins are of known structure. High-throughput crystallography offers …
Changes in membrane protein structural biology
J Birch, H Cheruvara, N Gamage, PJ Harrison, R Lithgo… - Biology, 2020 - mdpi.com
Simple Summary Membrane proteins are essential to all forms of life. Millions of membrane
proteins are found in the lipid membrane layer that surrounds cells, and in the lipid …
proteins are found in the lipid membrane layer that surrounds cells, and in the lipid …
Artificial membranes for membrane protein purification, functionality and structure studies
Membrane proteins represent one of the most important targets for pharmaceutical
companies. Unfortunately, technical limitations have long been a major hindrance in our …
companies. Unfortunately, technical limitations have long been a major hindrance in our …
[图书][B] Membrane protein purification and crystallization: a practical guide
C Hunte, G Von Jagow, H Schagger - 2003 - books.google.com
This second edition of Membrane Protein Purification and Crystallization, A Practical Guide
is written for bench scientists working in the fields of biochemistry, biology, and proteomic …
is written for bench scientists working in the fields of biochemistry, biology, and proteomic …
The recombinant expression systems for structure determination of eukaryotic membrane proteins
Y He, K Wang, N Yan - Protein & cell, 2014 - academic.oup.com
Eukaryotic membrane proteins, many of which are key players in various biological
processes, constitute more than half of the drug targets and represent important candidates …
processes, constitute more than half of the drug targets and represent important candidates …
Strategies for the purification of membrane proteins
SM Smith - Protein Chromatography: Methods and Protocols, 2017 - Springer
Although membrane proteins account for approximately 30% of the coding regions of all
sequenced genomes and play crucial roles in many fundamental cell processes, there are …
sequenced genomes and play crucial roles in many fundamental cell processes, there are …
Overcoming bottlenecks in the membrane protein structural biology pipeline
D Hardy, RM Bill, A Jawhari… - Biochemical Society …, 2016 - portlandpress.com
Membrane proteins account for a third of the eukaryotic proteome, but are greatly under-
represented in the Protein Data Bank. Unfortunately, recent technological advances in X-ray …
represented in the Protein Data Bank. Unfortunately, recent technological advances in X-ray …