Current strategies for protein production and purification enabling membrane protein structural biology

A Pandey, K Shin, RE Patterson… - Biochemistry and Cell …, 2016 - cdnsciencepub.com
Membrane proteins are still heavily under-represented in the protein data bank (PDB),
owing to multiple bottlenecks. The typical low abundance of membrane proteins in their …

[HTML][HTML] Overcoming the challenges of membrane protein crystallography

EP Carpenter, K Beis, AD Cameron, S Iwata - Current opinion in structural …, 2008 - Elsevier
Membrane protein structural biology is still a largely unconquered area, given that
approximately 25% of all proteins are membrane proteins and yet less than 150 unique …

Membrane protein structural biology–how far can the bugs take us?

E Granseth, S Seppälä, M Rapp, DO Daley… - Molecular membrane …, 2007 - Taylor & Francis
Membrane proteins are core components of many essential cellular processes, and high-
resolution structural data is therefore highly sought after. However, owing to the many …

Membrane protein structural biology: the high throughput challenge

PJ Loll - Journal of structural biology, 2003 - Elsevier
Membrane proteins represent roughly one-third of the proteins encoded in the genome, yet
fewer than 1% of the proteins are of known structure. High-throughput crystallography offers …

Changes in membrane protein structural biology

J Birch, H Cheruvara, N Gamage, PJ Harrison, R Lithgo… - Biology, 2020 - mdpi.com
Simple Summary Membrane proteins are essential to all forms of life. Millions of membrane
proteins are found in the lipid membrane layer that surrounds cells, and in the lipid …

Artificial membranes for membrane protein purification, functionality and structure studies

MJ Parmar, CDM Lousa, SP Muench… - Biochemical Society …, 2016 - portlandpress.com
Membrane proteins represent one of the most important targets for pharmaceutical
companies. Unfortunately, technical limitations have long been a major hindrance in our …

[图书][B] Membrane protein purification and crystallization: a practical guide

C Hunte, G Von Jagow, H Schagger - 2003 - books.google.com
This second edition of Membrane Protein Purification and Crystallization, A Practical Guide
is written for bench scientists working in the fields of biochemistry, biology, and proteomic …

The recombinant expression systems for structure determination of eukaryotic membrane proteins

Y He, K Wang, N Yan - Protein & cell, 2014 - academic.oup.com
Eukaryotic membrane proteins, many of which are key players in various biological
processes, constitute more than half of the drug targets and represent important candidates …

Strategies for the purification of membrane proteins

SM Smith - Protein Chromatography: Methods and Protocols, 2017 - Springer
Although membrane proteins account for approximately 30% of the coding regions of all
sequenced genomes and play crucial roles in many fundamental cell processes, there are …

Overcoming bottlenecks in the membrane protein structural biology pipeline

D Hardy, RM Bill, A Jawhari… - Biochemical Society …, 2016 - portlandpress.com
Membrane proteins account for a third of the eukaryotic proteome, but are greatly under-
represented in the Protein Data Bank. Unfortunately, recent technological advances in X-ray …