A procedure for the nonradioactive labeling of oligonucleotides with the hapten digoxigenin
(DIG) has been developed. The label is introduced by enzymatic tailing of the 3′-end. Two …

We have developed a system for the enzymatic in vitro synthesis of non-radioactively
labeled RNA which is derivatized with the hapten digoxigenin (DIG). The labeling reaction …

The use of non-radioactive nucleic acid probes has increased dramatically over the last
years. The convenience of not having to deal with radioactive isotopes combined with the …

Probes nonradioactively labeled with the steroid hapten digoxigenin have serveral intriguing
properties, including a high sensitivity equivalent to that of radioactive probes, speed in …

The digoxigenin-based non-radioactive DNA labeling and detection system was applied in
various hybridization protocols using digoxigeninlabeled probes obtained by enzymatic …

A novel highly sensitive non-radioactive DNA labeling and detection system based on the
ELISA principle has been developed. DNA is modified with the cardenolide-hapten …

The random-primed DNA labeling technique was modified for the incorporation of
digoxigenin into DNA as basic component of the digoxigeninbased non-radioactive DNA …

A fast and simple protocol for the chemiluminescent detection of digoxigenin-labeled nucleic
acids with anti-digoxigenin antibody Fab fragments coupled to alkaline phosphatase and 3 …

A review is given on the novel non-radioactive digoxigenin: anti-digoxigenin (DIG)
bioanalytical indicator system. After a general introduction on direct and indirect indicator …

A highly sensitive method for detecting specific nucleotide sequences was recently
developed. The method uses digoxigenin-labeled nucleic acid probes for hybridization to …