A system for rapid DNA sequencing with fluorescent chain-terminating dideoxynucleotides
JM Prober, GL Trainor, RJ Dam, FW Hobbs… - Science, 1987 - science.org
A DNA sequencing system based on the use of a novel set of four chain-terminating
dideoxynucleotides, each carrying a different chemically tuned succinylfluorescein dye …
dideoxynucleotides, each carrying a different chemically tuned succinylfluorescein dye …
Multiplex DNA sequencing
GM Church, S Kieffer-Higgins - Science, 1988 - science.org
The increasing demand for DNA sequences can be met by replacement of each DNA
sample in a device with a mixture of N samples so that the normal throughput is increased …
sample in a device with a mixture of N samples so that the normal throughput is increased …
Fluorescence detection in automated DNA sequence analysis
LM Smith, JZ Sanders, RJ Kaiser, P Hughes, C Dodd… - Nature, 1986 - nature.com
We have developed a method for the partial automation of DNA sequence analysis.
Fluorescence detection of the DNA fragments is accomplished by means of a fluorophore …
Fluorescence detection of the DNA fragments is accomplished by means of a fluorophore …
Shotgun sequencing of the human genome
The Human Genome Project (HGP) was officially launched in the United States on 1 October
1990 as a 15-year program to map and sequence the complete set of human chromosomes …
1990 as a 15-year program to map and sequence the complete set of human chromosomes …
Electrophoretically uniform fluorescent dyes for automated DNA sequencing
ML Metzker, J Lu, RA Gibbs - Science, 1996 - science.org
A class of dyes, BODIPY fluorophores, has been identified for automated DNA sequencing
that has improved spectral characteristics compared with conventional fluorescein and …
that has improved spectral characteristics compared with conventional fluorescein and …
Use of unpurified synthetic deoxynucleotide primers for rapid dideoxynucleotide chain termination sequencing
RAY SANCHEZ-PESCADOR, MS URDEA - DNA, 1984 - liebertpub.com
Synthetic oligodeoxyribonucleotides were used as primers for sequencing DNA by the
dideoxy chain termination method. We report that the use of unpurified preparations of such …
dideoxy chain termination method. We report that the use of unpurified preparations of such …
Specific-primer-directed DNA sequencing
EC Strauss, JA Kobori, G Siu, LE Hood - Analytical biochemistry, 1986 - Elsevier
A simple and rapid strategy for DNA sequence analysis based on the Sanger chain-
termination method is described. This procedure utilizes full-sized inserts of 1 to 4 kb of DNA …
termination method is described. This procedure utilizes full-sized inserts of 1 to 4 kb of DNA …
The future of DNA sequencing
LM Smith - Science, 1993 - science.org
Lloyd M. Smith cross-linked solutions are liquid, albeit vis-cous, and thus may in principle be
pumped in and out of theelectrophoresis cell as desired. They offer for the first time the possi …
pumped in and out of theelectrophoresis cell as desired. They offer for the first time the possi …
Direct cloning and sequence analysis of enzymatically amplified genomic sequences
SJ Scharf, GT Horn, HA Erlich - Science, 1986 - science.org
A method is described for directly cloning enzymatically amplified segments of genomic
DNA into an M13 vector for sequence analysis. A 110-base pair fragment of the human β …
DNA into an M13 vector for sequence analysis. A 110-base pair fragment of the human β …
Continuous, on-line DNA sequencing using oligodeoxynucleotide primers with multiple fluorophores.
JA Brumbaugh, LR Middendorf… - Proceedings of the …, 1988 - National Acad Sciences
A method for sequencing DNA by using a difluoresceinated primer and laser excitation is
described. Dideoxy protocols have been determined that provide sequences for 600 bases …
described. Dideoxy protocols have been determined that provide sequences for 600 bases …