Further refinement of culture media is needed to improve the quality of embryos generated
in vitro. Previous results from our laboratory demonstrated that uptake of nutrients by the …

The cleavage, metabolism and viability of mouse zygotes were assessed after culture in
media of different ionic and metabolite composition. Medium with a high potassium …

Recent studies in our laboratory have indicated that bovine embryos only use a small
amount of the nutrients available to them in culture. Our objective was to evaluate the …

Background Retarded embryo growth is a pervasive effect of culture in vitro. Methods A
systematic analysis of the interactions between media design, embryo culture density …

After compaction, the preimplantation mouse embryo switches to a glucose-based
metabolism, whereas for the 2-to 4-cell stage embryo, glucose can be inhibitory. In this …

Preliminary observations showed that one-cell embryos from random-bred MF1 mice avoid
cleavage arrest at the two-cell stage ('in vitro two-cell block') when cultured in modified M16 …

Efficient model production in rats that incorporates newly developed genetic editing and
embryo transfer tools, such as CRISPR/Cas9 technology and non-surgical embryo transfer …

The introduction of stress to embryonic blastomeres through inappropriate culture conditions
results ultimately in the loss of viability. Retention of normal metabolic function in human …

Objective To determine compositions of commercial single-step culture media and test with
a murine model whether differences in composition are biologically relevant. Design …

In experiment 1, the effects of a group of either 20 (ie glutamine+ essential+ non-essential)
or 11 (ie hamster embryo culture medium (HECM)-6) amino acids were evaluated in …