Tetracycline (TC)-sensing bioreporters using green fluorescent protein (GFP) were
generated in Escherichia coli and solvent-tolerant Acinetobacter oleivorans. A TC-inducible …

Three different mini-Tn 5 plasmids, containing a tetracycline-inducible promoter, P tet and a
regulatory gene, tetR, in operon fusions with a reporter gene system (lacZYA, luxCDABE or …

An extended range whole-cell tetracycline biosensor strain was constructed by insertion of
the tet (M) gene, encoding tetracycline resistance by ribosomal protection, into plasmid …

Three separate genetic strategies, based upon the induced expression of three different
genes (lacZ, selA and nuoA) were tested to provide the SciTox assay with sensitive and …

A plasmid containing a transcriptional fusion between fet regulated fet promoter from
plasmid pOT182 and Enhanced Green Fluorescent Protein (EGFP) gene was created and …

Materials and Methods Mouse embryo fibroblast 10T1/2 cells in a 35 mm culture dish were
transfected with 2 μg of pcDNA6/TR plasmid DNA (Invitrogen, Carlsbad, CA, USA) with …

A sensitive microbiological detection system for tetracyclines, utilizing an Escherichia coli
strain containing a cloned tetA-lacZ gene fusion, is described. Expression of beta …

A bioluminescent Escherichia coli K-12 strain for the specific detection of the tetracycline
group of antibiotics is described. A sensor plasmid, containing five genes from bacterial …

Antibiotic abuse is the main reason for the drug resistance of pathogenic bacteria, posing a
potential health risk. Antibiotic surveillance is critical for preventing antibiotic contamination …

The performance of two bioluminescent Escherichia coli K‐12 strains for the specific
detection of the tetracycline family of antimicrobial agents was compared, and the analytical …