Highlights•Compares and contrasts new affinity methods to dominant conventional
methods.•Explains how the popular His tag, despite its age, still dominates this …

The affinity tags are unique proteins/peptides that are attached at the N-or C-terminus of the
recombinant proteins. These tags help in protein purification. Additionally, some affinity tags …

Affinity tags are highly efficient tools for protein purification. They allow the purification of
virtually any protein without any prior knowledge of its biochemical properties. The use of …

Tag-assisted protein purification is a method of choice for both academic researches and
large-scale industrial demands. Application of the purification tags in the protein production …

When the first version of this unit was written in 1995, protein purification of recombinant
proteins was based on a variety of standard chromatographic methods and approaches …

Protein purification remains a central need for biotechnology. In recent years, a class of
aggregating tags has emerged, which offers a quick, cost‐effective and column‐free …

The purification of recombinant proteins by affinity chromatography is one of the most
efficient strategies due to the high recovery yields and purity achieved. However, this is …

Affinity tags are highly efficient tools for purifying proteins from crude extracts. To facilitate
the selection of affinity tags for purification projects, we have compared the efficiency of eight …

We introduce a new method for purifying recombinant proteins expressed in bacteria using a
highly specific, inducible, self-cleaving protease tag. This tag is comprised of the Vibrio …

Conventional column chromatography processes to purify recombinant proteins are
associated with high production costs and slow volumetric throughput at both laboratory and …